Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Anal Biochem. 2011 Aug 1;415(1):59-66. doi: 10.1016/j.ab.2011.04.003. Epub 2011 Apr 15.
A high-resolution method for the separation and analysis of disaccharides prepared from heparin and heparan sulfate (HS) using heparin lyases is described. Ultra-performance liquid chromatography in a reverse-phase ion-pairing mode efficiently separates eight heparin/HS disaccharides. The disaccharides can then be detected and quantified using electrospray ionization mass spectrometry. This method is particularly useful in the analysis of small amounts of biological samples, including cells, tissues, and biological fluids, because it provides high sensitivity without being subject to interference from proteins, peptides, and other sample impurities.
本文描述了一种使用肝素酶从肝素和硫酸乙酰肝素（HS）制备的二糖的高分辨率分离和分析方法。反相离子对高效液相色谱法可有效分离八种肝素/HS 二糖。然后，可使用电喷雾电离质谱法对二糖进行检测和定量。该方法在分析少量生物样品（包括细胞、组织和生物体液）时特别有用，因为它具有高灵敏度，并且不受蛋白质、肽和其他样品杂质的干扰。
