Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.
Anal Biochem. 2010 Feb 1;397(1):24-8. doi: 10.1016/j.ab.2009.09.031. Epub 2009 Sep 19.
Chondroitin sulfate (CS) has an important role in cell division, in the central nervous system, and in joint-related pathologies such as osteoarthritis. Due to the complex chemical structure and biological importance of CS, simple, sensitive, high resolution, and robust analytical methods are needed for the analysis of CS disaccharides and oligosaccharides. An ion-pairing, reversed-phase, ultraperformance liquid chromatography (IPRP-UPLC) separation, coupled to electrospray ionization mass spectrometry with an ion trap mass analyzer, was applied for the analyses of CS-derived disaccharides. UPLC separation technology uses small particle diameter, short column length, and elevated column temperature to obtain high resolution and sensitivity. Hexylamine (15 mM) was selected as the optimal ion-pairing reagent.
硫酸软骨素（CS）在细胞分裂、中枢神经系统以及与关节相关的疾病（如骨关节炎）中具有重要作用。由于 CS 的复杂化学结构和生物学重要性，需要简单、灵敏、高分辨率和稳健的分析方法来分析 CS 二糖和寡糖。本研究采用离子对反相超高效液相色谱（IPRP-UPLC）分离，结合带有离子阱质量分析仪的电喷雾电离质谱法，用于分析 CS 衍生的二糖。UPLC 分离技术采用小粒径、短柱长和升高的柱温来获得高分辨率和灵敏度。十六烷基胺（15mM）被选为最佳的离子对试剂。
