Department of Physiology, University of Saskatchewan, Saskatoon, Saskatchewan S7N3R2, Canada.
J Neurosci Methods. 2011 Jun 15;198(2):274-9. doi: 10.1016/j.jneumeth.2011.04.015. Epub 2011 Apr 16.
Here we introduce a simple experimental approach for studying afferent pain fibre physiology. We have developed a mouse en bloc dural-skull preparation for optical microfluorometric imaging to directly study the physiological functioning in selectively identified, individual nociceptive fibre free nerve endings. Functional optical imaging using widefield epifluorescence microscopy was combined with electrophysiological stimulations, pharmacological manipulations, and the UV photolysis of caged compounds. For the first time, we show high-resolution functional imaging of single action potential-evoked fluorescent transients, as well as sub- and supra-threshold calcium signaling events within individual nociceptive fibre terminations. This novel experimental approach opens up a new window for studying nociceptive fibre physiology and pathophysiology.
在这里，我们介绍一种简单的实验方法来研究传入痛觉纤维生理学。我们开发了一种用于光学微荧光成像的小鼠整块硬脑膜-颅骨制备方法，以直接研究选择性识别的、单个伤害感受纤维游离神经末梢的生理功能。使用宽场落射荧光显微镜的功能光学成像与电生理刺激、药理学操作以及笼状化合物的紫外线光解相结合。我们首次展示了单个动作电位诱发的荧光瞬变以及单个伤害感受纤维末端亚阈值和超阈值钙信号事件的高分辨率功能成像。这种新的实验方法为研究伤害感受纤维的生理学和病理生理学开辟了一个新的窗口。
