Batlle D C, Saleh A, Rombola G
Northwestern University Medical School, Chicago 60611.
Hypertension. 1990 Jan;15(1):97-103. doi: 10.1161/01.hyp.15.1.97.
This study was designed to determine the cytoplasmic pH (pHi) profile of lymphocytes from a rat model of genetic hypertension that is well suited for study before and after the development of spontaneous hypertension. For this purpose, pHi was measured in thymic lymphocytes obtained from spontaneously hypertensive rats (SHR) and from age-matched Wistar-Kyoto (WKY) control rats using 2',7'-bis carboxyethyl-5,6-carboxyfluorescein (BCECF), a pH-sensitive fluorescence probe. At the age of 16-20 weeks, pHi of lymphocytes suspended in a HCO3-free HEPES-buffered solution, was markedly lower in the SHR than in the WKY rats (7.07 +/- 0.02, n = 16 and 7.22 +/- 0.01, n = 15, respectively, p less than 0.001), whereas systolic blood pressure was higher in SHR than in WKY rats (175 +/- 5.0 and 105 +/- 3.0 mm Hg, respectively, p less than 0.001). In rats less than 5 weeks of age, pHi was also lower in SHR than in WKY rat lymphocytes (7.12 +/- 0.04, n = 11 and 7.23 +/- 0.04, n = 11, respectively, p less than 0.05), although at this age systolic blood pressure was not different between the two groups (87 +/- 4.0 and 85 +/- 3.0 mm Hg, respectively). In lymphocytes suspended in a more physiological HCO3/CO2-buffered solution, pHi was again lower in the adult SHR than in the WKY rat (7.18 +/- 0.02, n = 16 and 7.31 +/- 0.02, n = 16, respectively, p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在确定一种遗传性高血压大鼠模型中淋巴细胞的细胞质pH（pHi）情况，该模型非常适合在自发性高血压发生前后进行研究。为此，使用pH敏感荧光探针2',7'-双羧乙基-5,6-羧基荧光素（BCECF），测量了从自发性高血压大鼠（SHR）和年龄匹配的Wistar-Kyoto（WKY）对照大鼠获取的胸腺淋巴细胞中的pHi。在16 - 20周龄时，悬浮于无HCO3的HEPES缓冲溶液中的SHR淋巴细胞的pHi显著低于WKY大鼠（分别为7.07±0.02，n = 16和7.22±0.01，n = 15，p < 0.001），而SHR的收缩压高于WKY大鼠（分别为175±5.0和105±3.0 mmHg，p < 0.001）。在小于5周龄的大鼠中，SHR淋巴细胞的pHi也低于WKY大鼠淋巴细胞（分别为7.12±0.04，n = 11和7.23±0.04，n = 11，p < 0.05），尽管这个年龄段两组的收缩压没有差异（分别为87±4.0和85±3.0 mmHg）。在悬浮于更接近生理状态的HCO3/CO2缓冲溶液中的淋巴细胞中，成年SHR的pHi再次低于WKY大鼠（分别为7.18±0.02，n = 16和7.31±0.02，n = 16，p < 0.001）。（摘要截断于250字）
