Gholivand M B, Kashanian S, Peyman H, Roshanfekr H
Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran.
Eur J Med Chem. 2011 Jul;46(7):2630-8. doi: 10.1016/j.ejmech.2011.03.034. Epub 2011 Apr 5.
This work presents the interaction of two anthraquinones including quinizarin (1, 4-dihydroxyanthraquinone) and danthron (1, 8-dihydroxyanthraquinone) with CT-DNA in a mixture of Brittone-Robinson buffer (pH=7) with 50% of ethanol by UV-vis absorption, circular dichroism spectroscopic methods and viscosity measurements. The PCA and INDICES methods were used for predicting the number of light-absorbing components. Partially intercalative and hydrogen binding were found to be the dominant binding modes between anthraquinones and CT-DNA. The effect of ionic strength and pH on the behavior of the above system and also the interaction of anthraquinones with ds and ss-DNA were used to confirm the mentioned binding modes. The EQUISPEC software and nonlinear least-squares analysis were applied for binding constant determination.
本研究通过紫外可见吸收光谱、圆二色光谱法和粘度测量，研究了两种蒽醌（包括醌茜（1,4 - 二羟基蒽醌）和丹蒽醌（1,8 - 二羟基蒽醌））在含有50%乙醇的 Brittone - Robinson 缓冲液（pH = 7）中与 CT - DNA 的相互作用。采用主成分分析（PCA）和指标法预测吸光成分的数量。发现蒽醌与 CT - DNA 之间的主要结合模式为部分嵌入和氢键结合。通过离子强度和 pH 对上述体系行为的影响以及蒽醌与双链和单链 DNA 的相互作用来证实上述结合模式。使用 EQUISPEC 软件和非线性最小二乘法分析来测定结合常数。
