Istanbul Technical University, Department of Chemistry, Maslak 34469, Istanbul, Turkey.
Bioresour Technol. 2011 Jul;102(13):6783-90. doi: 10.1016/j.biortech.2011.03.050. Epub 2011 Mar 27.
Densely cross-linked poly(vinylamine) microbeads (∼ 2 μm) were prepared by precipitation copolymerization of N-vinyl formamide and ethylene glycoldimethacrylate in acetonitrile. The formamido groups of the microbeads were hydrolyzed into amino groups. Then, amino-functionalized microbeads were used for covalent immobilization of laccase via glutaraldehyde coupling. The average amount of immobilized enzyme was 18.7 mg/g microbeads. Kinetic parameters, V(max) and K(m) values were determined as 20.7 U/mg protein and 2.76 × 10(-2)mmol/L for free enzyme and 15.8 U/mg protein and 4.65 mmol/L for the immobilized laccase, respectively. The immobilized laccase was operated in a batch reactor for the degradation of two different benzidine based dyes (i.e., Direct Blue 1 and Direct Red 128). The laccase immobilized on the microbeads was very effective for removal of these dyes which interfere with the hormonal system.
通过在乙腈中的沉淀共聚反应，制备了交联度高的聚（乙烯亚胺）微球（∼2μm），其中包含 N-乙烯基甲酰胺和乙二醇二甲基丙烯酸酯。微球上的甲酰胺基被水解成氨基。然后，氨基化的微球用于通过戊二醛偶联共价固定化漆酶。固定化酶的平均量为 18.7mg/g 微球。分别测定了游离酶和固定化漆酶的动力学参数 Vmax 和 Km 值，分别为 20.7U/mg 蛋白和 2.76×10(-2)mmol/L，以及 15.8U/mg 蛋白和 4.65mmol/L。固定化漆酶在分批式反应器中用于两种不同的联苯胺基染料（即直接蓝 1 和直接红 128）的降解。固定在微球上的漆酶对去除这些干扰激素系统的染料非常有效。
