Lin Ming-Feng, Kuo Han-Yueh, Yeh Hui-Wen, Yang Chih-Man, Sung Chih-Hung, Tu Chi-Chao, Huang Mei-Luan, Liou Ming-Li
Department of Health, Chutung Hospital, Hsin-Chu County, Taiwan.
Department of Health, Hsin-Chu General Hospital, Hsin-Chu City, Taiwan.
J Microbiol Immunol Infect. 2011 Feb;44(1):39-44. doi: 10.1016/j.jmii.2011.01.008. Epub 2011 Jan 12.
The distribution and characterization of OXA-type carbapenemases in Acinetobacter sp in Taiwan has less been reported. The aim of the study was to investigate the molecular epidemiology and OXA-type carbapenemase genes in a regional hospital in Taiwan.
Imipenem-resistant Acinetobacter sp were collected between 2005 and 2007 in a regional hospital. Genotyping was performed by pulsed-field gel electrophoresis. OXA-type carbapenemase genes were determined by multiplex polymerase chain reaction (PCR) and gene sequencing.
A total of 136 isolates were collected. Fifty-six pulsotypes were identified. None of the pulsotypes established predominance throughout the 3-year period. Multiplex PCR of blaOXA genes showed that 99% (135/136) of the Acinetobacter sp possessed blaOXA51-like genes. The coexistences of blaOXA51-like/blaOXA-23-like and blaOXA51-like/blaOXA-24-like were detected in 19% (26/136) and 1% (2/136) of the isolates, respectively. Among blaOXA-23-like gene-carrying isolates, two isolates (Pulsotypes 18 and 20) were found in 2006 and the remainder (n=24), including Pulsotypes 27 (n=18), 29 (n=1), 52 (n=3), and 53 (n=2), were found in 2007. Sequencing performed on the 26 representative isolates confirmed the presence of the blaOXA-23 carbapenemase gene. Analysis of the genetic content of blaOXA-23 showed that these genes were presumably chromosomal and associated with the upstream-located insertion sequence ISAba1.
The emergence and imminent widespread of blaOXA-23-carrying imipenem-resistant Acinetobacter sp appeared in Taiwan during the period from 2006 to 2007.
台湾地区不动杆菌属中OXA型碳青霉烯酶的分布及特征鲜见报道。本研究旨在调查台湾某地区医院中不动杆菌属的分子流行病学及OXA型碳青霉烯酶基因。
于2005年至2007年在某地区医院收集耐亚胺培南不动杆菌属。采用脉冲场凝胶电泳进行基因分型。通过多重聚合酶链反应（PCR）和基因测序确定OXA型碳青霉烯酶基因。
共收集到136株分离株。鉴定出56种脉冲型。在这3年期间，没有一种脉冲型占主导地位。blaOXA基因的多重PCR显示，99%（135/136）的不动杆菌属拥有blaOXA51样基因。分别在19%（26/136）和1%（2/136）的分离株中检测到blaOXA51样/blaOXA - 23样和blaOXA51样/blaOXA - 24样的共存情况。在携带blaOXA - 23样基因的分离株中，2006年发现了2株（脉冲型18和20），其余（n = 24），包括脉冲型27（n = 18）、29（n = 1）、52（n = 3）和53（n = 2），于2007年发现。对26株代表性分离株进行测序证实存在blaOXA - 23碳青霉烯酶基因。对blaOXA - 23的遗传内容分析表明，这些基因可能位于染色体上，并与上游的插入序列ISAba1相关。
2006年至2007年期间，台湾出现了携带blaOXA - 23的耐亚胺培南不动杆菌属，并即将广泛传播。
