Institute of Infectious Disease and Molecular Medicine, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Anzio Road, Observatory 7925, South Africa.
Virology. 2011 Jul 5;415(2):107-13. doi: 10.1016/j.virol.2010.12.027. Epub 2011 May 4.
The Mbeya region of Tanzania has a genetically complex HIV epidemic with multiple subtypes and recombinant forms circulating, together with a high frequency of dual infections with more than one subtype. This study aimed to determine whether this impacted the HIV-1 transmission bottleneck. A total of 210 env sequences from 22 participants were generated from recently infected women from Mbeya using the single genome amplification approach. Participants were infected with subtypes C (n=9), A (n=4), or D (n=1), and recombinants AC (n=4), CD (n=2), AD (n=1), or ACD (n=1). Sixteen participants (73%) were infected with a single variant; five (23%) with multiple variants; and one (4%) was dually infected. Thus the frequency of single variant infections was similar to cohorts located in genetically restricted subtype B or C epidemics, suggesting that multiple circulating subtypes and unique recombinant forms do not have a significant impact on the transmission bottleneck.
坦桑尼亚姆贝亚地区的艾滋病毒流行具有遗传复杂性，多种亚型和重组形式循环存在，同时存在多种亚型的双重感染率很高。本研究旨在确定这是否会影响 HIV-1 传播瓶颈。采用单基因组扩增方法，从姆贝亚的 22 名新近感染的女性中获得了 210 个来自 210 名参与者的 env 序列。参与者感染了 C 型（n=9）、A 型（n=4）或 D 型（n=1），以及重组体 AC（n=4）、CD（n=2）、AD（n=1）或 ACD（n=1）。16 名参与者（73%）感染了单一变异体；5 名（23%）感染了多种变异体；1 名（4%）双重感染。因此，单一变异体感染的频率与位于遗传受限的 B 或 C 型流行区的队列相似，这表明多种循环亚型和独特的重组形式对传播瓶颈没有重大影响。
