假基因衍生的小干扰 RNA 调控非洲锥虫布鲁斯氏菌中的基因表达。
Pseudogene-derived small interference RNAs regulate gene expression in African Trypanosoma brucei.
机构信息
Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
出版信息
Proc Natl Acad Sci U S A. 2011 May 17;108(20):8345-50. doi: 10.1073/pnas.1103894108. Epub 2011 Apr 29.
Abstract
Pseudogenes have been shown to acquire unique regulatory roles from more and more organisms. We report the observation of a cluster of siRNAs derived from pseudogenes of African Trypanosoma brucei using high through-put analysis. We show that these pseudogene-derived siRNAs suppress gene expression through RNA interference. The discovery that siRNAs may originate from pseudogenes and regulate gene expression in a unicellular eukaryote provides insights into the functional roles of pseudogenes and into the origin of noncoding small RNAs.
摘要
越来越多的研究表明，假基因可以获得独特的调控作用。我们报道了使用高通量分析观察到来自非洲布氏锥虫假基因的一组 siRNA。我们表明，这些假基因衍生的 siRNA 通过 RNA 干扰抑制基因表达。siRNA 可能起源于假基因并在单细胞真核生物中调节基因表达的发现，为假基因的功能作用以及非编码小 RNA 的起源提供了新的见解。
相似文献
1
Pseudogene-derived small interference RNAs regulate gene expression in African Trypanosoma brucei.假基因衍生的小干扰 RNA 调控非洲锥虫布鲁斯氏菌中的基因表达。
Proc Natl Acad Sci U S A. 2011 May 17;108(20):8345-50. doi: 10.1073/pnas.1103894108. Epub 2011 Apr 29.
2
Comparative transcriptome analysis of small noncoding RNAs in different stages of Trypanosoma brucei.比较不同发育阶段布氏锥虫小非编码 RNA 的转录组分析。
RNA. 2013 Jul;19(7):863-75. doi: 10.1261/rna.035683.112. Epub 2013 May 23.
3
Pseudogene-derived small interfering RNAs regulate gene expression in mouse oocytes.假基因衍生的小干扰RNA调节小鼠卵母细胞中的基因表达。
Nature. 2008 May 22;453(7194):534-8. doi: 10.1038/nature06904. Epub 2008 Apr 10.
4
An siRNA ribonucleoprotein is found associated with polyribosomes in Trypanosoma brucei.在布氏锥虫中发现一种与多核糖体相关的小干扰RNA核糖核蛋白。
RNA. 2003 Jul;9(7):802-8. doi: 10.1261/rna.5270203.
5
Pseudogenes are not pseudo any more.假基因不再是“假”的了。
RNA Biol. 2012 Jan;9(1):27-32. doi: 10.4161/rna.9.1.18277. Epub 2012 Jan 1.
6
Not so pseudo anymore: pseudogenes as therapeutic targets.不再是假的了：假基因作为治疗靶点。
Pharmacogenomics. 2013 Dec;14(16):2023-34. doi: 10.2217/pgs.13.172.
7
In vivo analysis of the RNA interference mechanism in Trypanosoma brucei.布氏锥虫RNA干扰机制的体内分析
Methods. 2003 Aug;30(4):304-12. doi: 10.1016/s1046-2023(03)00047-1.
8
Small interfering RNA-producing loci in the ancient parasitic eukaryote Trypanosoma brucei.在古老的寄生真核生物布氏锥虫中产生小干扰 RNA 的基因座。
BMC Genomics. 2012 Aug 27;13:427. doi: 10.1186/1471-2164-13-427.
9
Argonaute protein in the early divergent eukaryote Trypanosoma brucei: control of small interfering RNA accumulation and retroposon transcript abundance.早期分化真核生物布氏锥虫中的AGO蛋白：对小干扰RNA积累和逆转座子转录本丰度的调控
Mol Cell Biol. 2004 Jan;24(1):420-7. doi: 10.1128/MCB.24.1.420-427.2004.
10
Small RNAs originated from pseudogenes: cis- or trans-acting?源自假基因的小RNA：顺式作用还是反式作用？
PLoS Comput Biol. 2009 Jul;5(7):e1000449. doi: 10.1371/journal.pcbi.1000449. Epub 2009 Jul 31.
引用本文的文献
1
Revisiting the functional annotation of TriTryp using sequence similarity tools.使用序列相似性工具重新审视锥虫属的功能注释。
Heliyon. 2024 Oct 11;10(20):e39243. doi: 10.1016/j.heliyon.2024.e39243. eCollection 2024 Oct 30.
2
Loss to gain: pseudogenes in microorganisms, focusing on eubacteria, and their biological significance.得失之间：微生物中的假基因，聚焦于真细菌及其生物学意义。
Appl Microbiol Biotechnol. 2024 May 8;108(1):328. doi: 10.1007/s00253-023-12971-w.
3
Pseudogenes in Cancer: State of the Art.癌症中的假基因：最新进展
Cancers (Basel). 2023 Aug 8;15(16):4024. doi: 10.3390/cancers15164024.
4
Enhanced genome-wide association reveals the role of YABBY11-NGATHA-LIKE1 in leaf serration development of Populus.增强全基因组关联揭示了 YABBY11-NGATHA-LIKE1 在杨树叶片锯齿发育中的作用。
Plant Physiol. 2023 Mar 17;191(3):1702-1718. doi: 10.1093/plphys/kiac585.
5
Current Status of Regulatory Non-Coding RNAs Research in the Tritryp.锥虫目调控性非编码RNA的研究现状
Noncoding RNA. 2022 Jul 18;8(4):54. doi: 10.3390/ncrna8040054.
6
Pseudogenes: Four Decades of Discovery.假基因：四十年的发现历程。
Methods Mol Biol. 2021;2324:3-18. doi: 10.1007/978-1-0716-1503-4_1.
7
Cancer, Retrogenes, and Evolution.癌症、逆转录基因与进化
Life (Basel). 2021 Jan 19;11(1):72. doi: 10.3390/life11010072.
8
Genomic Organization and Generation of Genetic Variability in the RHS (Retrotransposon Hot Spot) Protein Multigene Family in .[具体物种名称]中RHS（逆转录转座子热点）蛋白多基因家族的基因组组织与遗传变异性的产生
Genes (Basel). 2020 Sep 17;11(9):1085. doi: 10.3390/genes11091085.
9
Identification of Potential Diagnostic and Prognostic Pseudogenes in Hepatocellular Carcinoma Based on Pseudogene-miRNA-mRNA Competitive Network.基于假基因-miRNA-mRNA 竞争网络鉴定肝细胞癌中的潜在诊断和预后假基因。
Med Sci Monit. 2020 May 16;26:e921895. doi: 10.12659/MSM.921895.
10
Re-recognition of pseudogenes: From molecular to clinical applications.假基因的再识别：从分子到临床应用。
Theranostics. 2020 Jan 1;10(4):1479-1499. doi: 10.7150/thno.40659. eCollection 2020.
本文引用的文献
1
Integrative genomics viewer.整合基因组浏览器。
Nat Biotechnol. 2011 Jan;29(1):24-6. doi: 10.1038/nbt.1754.
2
Small nucleolar RNA interference in Trypanosoma brucei: mechanism and utilization for elucidating the function of snoRNAs.小核仁 RNA 干扰在布氏锥虫中的作用机制及 snoRNAs 功能阐明方面的应用
Nucleic Acids Res. 2010 Nov;38(20):7236-47. doi: 10.1093/nar/gkq599. Epub 2010 Jul 3.
3
Deep sequencing of human nuclear and cytoplasmic small RNAs reveals an unexpectedly complex subcellular distribution of miRNAs and tRNA 3' trailers.对人类核和细胞质小 RNA 的深度测序揭示了 miRNA 和 tRNA 3' 尾端在亚细胞水平分布的复杂性出人意料。
PLoS One. 2010 May 14;5(5):e10563. doi: 10.1371/journal.pone.0010563.
4
Coordinated gene expression by post-transcriptional regulons in African trypanosomes.非洲锥虫中转录后调控子对基因表达的协调作用
J Biol. 2009;8(11):100. doi: 10.1186/jbiol203. Epub 2009 Dec 14.
5
deepBase: a database for deeply annotating and mining deep sequencing data.深碱基数据库：用于深度注释和挖掘深度测序数据的数据库。
Nucleic Acids Res. 2010 Jan;38(Database issue):D123-30. doi: 10.1093/nar/gkp943. Epub 2009 Dec 4.
6
Transcriptome analysis of differentiating trypanosomes reveals the existence of multiple post-transcriptional regulons.对分化型锥虫的转录组分析揭示了多个转录后调控子的存在。
BMC Genomics. 2009 Oct 26;10:495. doi: 10.1186/1471-2164-10-495.
7
TriTrypDB: a functional genomic resource for the Trypanosomatidae.TriTrypDB：锥虫科的功能基因组资源。
Nucleic Acids Res. 2010 Jan;38(Database issue):D457-62. doi: 10.1093/nar/gkp851. Epub 2009 Oct 20.
8
Widespread variation in transcript abundance within and across developmental stages of Trypanosoma brucei.布氏锥虫发育阶段内及不同发育阶段间转录本丰度存在广泛差异。
BMC Genomics. 2009 Oct 19;10:482. doi: 10.1186/1471-2164-10-482.
9
Human African trypanosomiasis.非洲人类锥虫病。
Lancet. 2010 Jan 9;375(9709):148-59. doi: 10.1016/S0140-6736(09)60829-1. Epub 2009 Oct 14.
10
Distinct and overlapping roles for two Dicer-like proteins in the RNA interference pathways of the ancient eukaryote Trypanosoma brucei.两种类Dicer蛋白在古老真核生物布氏锥虫RNA干扰途径中的不同及重叠作用
Proc Natl Acad Sci U S A. 2009 Oct 20;106(42):17933-8. doi: 10.1073/pnas.0907766106. Epub 2009 Oct 6.
Zotero 插件