Department of Microbiology and Institute of Basic Science, Dankook University, Cheonan 330-714, Korea.
J Microbiol Biotechnol. 2011 Apr;21(4):387-90.
Nude mice (BALB/c) were grafted with human 293 cells and PERV (porcine endogenous retrovirus)-IRES-EGFP (a packageable retroviral vector plasmid containing an internal ribosome entry site-enhanced green fluorescent protein)-producing pig PK15 cells in order to determine whether the pig cells could transmit PERV-IRES-EGFP to mice and human 293 cells in vivo. None of the transplanted human 293 cell lines were infected by PERV, but PCR analysis identified PERV-B provirus integration into both the heart and salivary gland of the inoculated nude mice. Our data indicate that hearts and salivary glands can be used to identify PERV-B receptors.
为了确定猪细胞能否在体内将 PERV-IRES-EGFP 传播给小鼠和人 293 细胞，将携带 PERV（猪内源性逆转录病毒）-IRES-EGFP（一种可包装的逆转录病毒载体质粒，含有内部核糖体进入位点增强型绿色荧光蛋白）的人 293 细胞和 PERV 产生的猪 PK15 细胞移植到裸鼠体内。没有任何移植的人 293 细胞系被 PERV 感染，但 PCR 分析鉴定出 PERV-B 前病毒整合到接种裸鼠的心脏和唾液腺中。我们的数据表明，心脏和唾液腺可用于鉴定 PERV-B 受体。
