阳离子脂质体（Lipofectin）在缺乏特异性受体的情况下介导逆转录病毒感染。
Cationic liposomes (Lipofectin) mediate retroviral infection in the absence of specific receptors.
作者信息
Innes C L, Smith P B, Langenbach R, Tindall K R, Boone L R
机构信息
Cellular and Genetic Toxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709.
出版信息
J Virol. 1990 Feb;64(2):957-61. doi: 10.1128/JVI.64.2.957-961.1990.
Abstract
We have used cationic liposomes (Lipofectin) to facilitate retrovirus infection of cells lacking the homologous viral receptor. Ecotropic murine leukemia virus and packaged retroviral vectors were shown to infect mink cells, and amphotropic packaged retroviral vectors were shown to infect hamster cells in the presence of Lipofectin but not in the presence of Polybrene. Lipofectin-mediated infection of cells lacking the homologous receptor results in a titer approximately 0.1% of the titer in cells with the homologous receptor, using the standard Polybrene protocol. The use of Lipofectin may provide a simple means to experimentally infect a wide variety of cells with viruses not normally infectious for the species, tissue, or cell type of interest.
摘要
我们使用阳离子脂质体（Lipofectin）来促进缺乏同源病毒受体的细胞的逆转录病毒感染。嗜亲性鼠白血病病毒和包装的逆转录病毒载体在Lipofectin存在下可感染貂细胞，而兼嗜性包装的逆转录病毒载体在Lipofectin存在下可感染仓鼠细胞，但在聚凝胺存在下则不能。使用标准的聚凝胺方案，Lipofectin介导的缺乏同源受体的细胞感染所产生的滴度约为具有同源受体的细胞中滴度的0.1%。使用Lipofectin可能提供一种简单的方法，用于通过通常对感兴趣的物种、组织或细胞类型无感染性的病毒来实验性地感染多种细胞。
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