Kozak C A, Gromet N J, Ikeda H, Buckler C E
Proc Natl Acad Sci U S A. 1984 Feb;81(3):834-7. doi: 10.1073/pnas.81.3.834.
Several strains of laboratory and wild-derived mice from Japan carry the dominant allele at the Fv-4 locus (Fv-4r) that is responsible for resistance to infection by exogenous ecotropic murine leukemia virus. We have used blot hybridization with a probe specific for the ecotropic viral envelope to show that a unique envelope-reactive sequence is present in the Japanese mouse Mus musculus molossinus and in four independently derived partially congeneic strains carrying Fv-4r. Analysis of 31 backcross mice shows complete concordance between inheritance of this fragment and resistance to Friend virus complex-induced erythroblastosis. Inheritance of this sequence also suppresses spontaneous expression of the endogenous ecotropic viruses carried by M. m. molossinus. Restriction enzyme analysis shows that the Fv-4r-associated sequence is different from the full-length ecotropic proviruses of laboratory mice. Infectious virus cannot be induced from mice carrying only the Fv-4r-associated sequence. Examination of other wild-derived mice resistant to Friend virus complex shows that Mus cervicolor cervicolor also contains the Fv-4r sequence. Our data indicate that a unique or incomplete provirus containing ecotropic envelope-related sequences is responsible for Fv-4-mediated resistance to both exogenous and endogenous ecotropic virus in various Asian mice.
来自日本的几种实验室小鼠品系和野生衍生小鼠品系在Fv-4位点携带显性等位基因（Fv-4r），该等位基因负责对外源亲嗜性鼠白血病病毒感染的抗性。我们使用了针对亲嗜性病毒包膜的特异性探针进行印迹杂交，以表明在日本小鼠小家鼠（Mus musculus molossinus）和四个独立衍生的携带Fv-4r的部分同源品系中存在独特的包膜反应性序列。对31只回交小鼠的分析表明，该片段的遗传与对弗氏病毒复合物诱导的成红细胞增多症的抗性完全一致。该序列的遗传也抑制了小家鼠携带的内源性亲嗜性病毒的自发表达。限制性酶切分析表明，与Fv-4r相关的序列不同于实验室小鼠的全长亲嗜性前病毒。仅携带与Fv-4r相关序列的小鼠不能诱导出感染性病毒。对其他对弗氏病毒复合物有抗性的野生衍生小鼠的检查表明，白腹鼠（Mus cervicolor cervicolor）也含有Fv-4r序列。我们的数据表明，含有亲嗜性包膜相关序列的独特或不完整前病毒负责Fv-4介导的各种亚洲小鼠对外源和内源性亲嗜性病毒的抗性。
