利用流式细胞术分析染色体中的重复 DNA。
Analysis of repetitive DNA in chromosomes by flow cytometry.
机构信息
Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.
出版信息
Nat Methods. 2011 Jun;8(6):484-6. doi: 10.1038/nmeth.1601. Epub 2011 May 1.
Abstract
We developed a flow cytometry method, chromosome flow fluorescence in situ hybridization (FISH), called CFF, to analyze repetitive DNA in chromosomes using FISH with directly labeled peptide nucleic acid (PNA) probes. We used CFF to measure the abundance of interstitial telomeric sequences in Chinese hamster chromosomes and major satellite sequences in mouse chromosomes. Using CFF we also identified parental homologs of human chromosome 18 with different amounts of repetitive DNA.
摘要
我们开发了一种流式细胞术方法，称为 CFF，即染色体流式荧光原位杂交，用于使用直接标记的肽核酸（PNA）探针进行 FISH 分析染色体中的重复 DNA。我们使用 CFF 测量中国仓鼠染色体中的端粒间序列和小鼠染色体中的主要卫星序列的丰度。我们还使用 CFF 鉴定了具有不同重复 DNA 量的人类 18 号染色体的亲本同源物。
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