Lansdorp P M, Verwoerd N P, van de Rijke F M, Dragowska V, Little M T, Dirks R W, Raap A K, Tanke H J
Terry Fox Laboratory, B.C. Cancer Agency, Vancouver, Canada.
Hum Mol Genet. 1996 May;5(5):685-91. doi: 10.1093/hmg/5.5.685.
Vertebrate chromosomes terminate in variable numbers of T2AG3 nucleotide repeats. In order to study telomere repeats at individual chromosomes, we developed novel, quantitative fluorescence in situ hybridization procedures using labeled (C3TA2)3 peptide nucleic acid and digital imaging microscopy. Telomere fluorescence intensity values from metaphase chromosomes of cultured human hematopoietic cells decreased with the replication history of the cells, varied up to six-fold within a metaphase, and were similar between sister chromatid telomeres. Surprisingly, telomere fluorescence intensity values within normal adult bone marrow metaphases did not show a normal distribution, suggesting that a minimum number of repeats at each telomere is required and/or maintained during normal hematopoiesis.
脊椎动物的染色体末端有数量可变的T2AG3核苷酸重复序列。为了研究单个染色体上的端粒重复序列，我们开发了新颖的定量荧光原位杂交方法，使用标记的（C3TA2）3肽核酸和数字成像显微镜。培养的人类造血细胞中期染色体的端粒荧光强度值随细胞的复制历史而降低，在一个中期内变化高达六倍，并且姐妹染色单体端粒之间相似。令人惊讶的是，正常成人骨髓中期的端粒荧光强度值未显示出正态分布，这表明在正常造血过程中每个端粒需要和/或维持最少数量的重复序列。
