Terry Fox Laboratory, BC Cancer Agency, Vancouver, BC V5Z 1L3, Canada.
European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, 9713 AV Groningen, the Netherlands.
Cell Rep Methods. 2022 Jan 24;2(1):100150. doi: 10.1016/j.crmeth.2021.100150.
Single-cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes, and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one-pot (OP) Strand-seq library preparation protocol in which reagents are added cumulatively, DNA purification steps are avoided, and enzymes are inactivated with a thermolabile protease. OP-Strand-seq libraries capture 10%-25% of the genome from a single-cell with reduced costs and increased throughput.
单细胞链测序产生定向基因组信息，可用于研究 DNA 修复、组装基因组以及将结构变异映射到染色体长度单倍型上。我们报告了一种纳升体积的、一管式（OP）链测序文库制备方案，其中试剂是累积添加的，避免了 DNA 纯化步骤，并且用热不稳定蛋白酶使酶失活。OP 链测序文库从单个细胞中捕获 10%-25%的基因组，成本降低，通量增加。
