淋病奈瑟菌中一个编码糖脂结合黏附素的基因的鉴定与特性分析。

Identification and characterization of a Neisseria gonorrhoeae gene encoding a glycolipid-binding adhesin.

作者信息

Paruchuri D K, Seifert H S, Ajioka R S, Karlsson K A, So M

机构信息

Department of Molecular Biology, Scripps Clinic and Research Foundation, La Jolla, CA 92037.

出版信息

Proc Natl Acad Sci U S A. 1990 Jan;87(1):333-7. doi: 10.1073/pnas.87.1.333.

We recently identified a set of mammalian cell receptors for Neisseria gonorrhoeae that are glycolipids. These receptors, lactosylceramide [Gal(beta 1-4)Glc(beta 1-1)Cer], gangliotriosylceramide [GalNAc( beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], and gangliotetraosylceramide [Gal(beta 1-3)GalNAc(beta 1-4)Gal(beta 1-4)Glc(beta 1-1)Cer], were shown to be specifically bound by a gonococcal outer membrane protein distinct from pilin and protein II. Here we report the isolation of the gene encoding the gangliotetraosylceramide-binding adhesin from a N. gonorrhoeae MS11 gene bank in Escherichia coli. Transposon mutagenesis studies in E. coli indicate that the adhesion is a protein with a molecular mass of 36,000 Da. The gene encoding the 36-kDa protein is duplicated in MS11 since two transposon insertions were required to abolish expression of the gene in this bacterium. This protein is present on the surface of the gonococcus and is not associated with the pilus.

我们最近鉴定出一组淋病奈瑟菌的哺乳动物细胞受体，它们是糖脂。这些受体，乳糖神经酰胺[半乳糖（β1-4）葡萄糖（β1-1）神经酰胺]、神经节三糖神经酰胺[N-乙酰神经氨酸（β1-4）半乳糖（β1-4）葡萄糖（β1-1）神经酰胺]和神经节四糖神经酰胺[半乳糖（β1-3）N-乙酰神经氨酸（β1-4）半乳糖（β1-4）葡萄糖（β1-1）神经酰胺]，被证明能被一种不同于菌毛蛋白和蛋白II的淋球菌外膜蛋白特异性结合。在此我们报告从大肠杆菌中的淋病奈瑟菌MS11基因文库中分离出编码神经节四糖神经酰胺结合黏附素的基因。在大肠杆菌中进行的转座子诱变研究表明，该黏附素是一种分子量为36,000道尔顿的蛋白质。编码36 kDa蛋白质的基因在MS11中是重复的，因为需要两次转座子插入才能消除该基因在这种细菌中的表达。这种蛋白质存在于淋球菌表面，与菌毛无关。