鉴定大麦（Hordeum vulgare L.）中的植酸酶基因。

BACKGROUND

Endogenous phytase plays a crucial role in phytate degradation and is thus closely related to nutrient efficiency in barley products. The understanding of genetic information of phytase in barley can provide a useful tool for breeding new barley varieties with high phytase activity.

METHODOLOGY/PRINCIPAL FINDINGS: Quantitative trait loci (QTL) analysis for phytase activity was conducted using a doubled haploid population. Phytase protein was purified and identified by the LC-ESI MS/MS Shotgun method. Purple acid phosphatase (PAP) gene was sequenced and the position was compared with the QTL controlling phytase activity. A major QTL for phytase activity was mapped to chromosome 5 H in barley. The gene controlling phytase activity in the region was named as mqPhy. The gene HvPAP a was mapped to the same position as mqPhy, supporting the colinearity between HvPAP a and mqPhy.

CONCLUSIONS/SIGNIFICANCE: It is the first report on QTLs for phytase activity and the results showed that HvPAP a, which shares a same position with the QTL, is a major phytase gene in barley grains.

背景

内源性植酸酶在植酸降解中起着至关重要的作用，因此与大麦产品中的营养效率密切相关。了解大麦中植酸酶的遗传信息可为培育具有高植酸酶活性的新型大麦品种提供有用工具。

方法/主要发现：使用双单倍体群体进行植酸酶活性的数量性状位点（QTL）分析。通过 LC-ESI MS/MS Shotgun 方法对植酸酶蛋白进行纯化和鉴定。对紫色酸性磷酸酶（PAP）基因进行测序，并将其位置与控制植酸酶活性的 QTL 进行比较。大麦 5H 染色体上定位了一个控制植酸酶活性的主要 QTL。该区域控制植酸酶活性的基因被命名为 mqPhy。基因 HvPAPa 与 mqPhy 定位在同一位置，支持 HvPAPa 和 mqPhy 之间的共线性。

结论/意义：这是首次报道植酸酶活性的 QTL ，结果表明，与 QTL 共享同一位置的 HvPAPa 是大麦籽粒中的主要植酸酶基因。