Koerten H K, Hazekamp J, Kroon M, Daems W T
Laboratory for Electron Microscopy, University of Leiden, The Netherlands.
Am J Pathol. 1990 Jan;136(1):141-57.
This report describes the cell biology of the development of asbestos bodies after a single intraperitoneal injection of a suspension of crocidolite asbestos fibers into the mouse peritoneal cavity. The majority of the infected fibers were found in aggregates of peritoneal macrophages, exudate cells, and fibrous tissue. These aggregates developed into granulomas containing not only numerous asbestos fibers, but also cells of various types, including macrophages, multinucleated giant cells, fibroblasts, plasma cells, granulocytes, and mast cells. Cytoplasmic ferritin was abundantly present in macrophages and giant cells. In addition, iron-rich inclusion bodies were detected. The results of this study show that asbestos body formation can occur outside the pleural cavity. Asbestos body formation occurred in the granulomas after periods of 1 month and longer. On the basis of morphologic criteria, various types of asbestos body were distinguished. X-ray microanalysis showed that variations in the density of the coat could attributed to the presence of chemical elements in various concentrations. Evidence is presented that asbestos body formation is an extracellular phenomenon.
本报告描述了将青石棉纤维悬浮液单次腹腔注射到小鼠腹腔后石棉小体形成的细胞生物学过程。大多数被感染的纤维存在于腹膜巨噬细胞、渗出细胞和纤维组织的聚集体中。这些聚集体发展成肉芽肿，其中不仅含有大量石棉纤维，还含有各种类型的细胞，包括巨噬细胞、多核巨细胞、成纤维细胞、浆细胞、粒细胞和肥大细胞。巨噬细胞和巨细胞的细胞质中大量存在铁蛋白。此外，还检测到富含铁的包涵体。本研究结果表明，石棉小体的形成可发生在胸腔外。石棉小体在1个月及更长时间后在肉芽肿中形成。根据形态学标准，区分了各种类型的石棉小体。X射线微分析表明，包被密度的变化可归因于不同浓度化学元素的存在。有证据表明石棉小体的形成是一种细胞外现象。
