Muranaka Toshiya
Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Osaka 565-0871, Japan.
Methods Mol Biol. 2011;744:27-35. doi: 10.1007/978-1-61779-123-9_3.
Gateway(®) system takes advantage of high-throughput creation of various expression vectors from one entry vector. This technology is also applied to RNAi vectors for functional analysis of plant genomics. To date, several plant Gateway vectors have been developed and distributed to plant science community. Here I would like to introduce unique plant Gateway vectors developed for functional analysis of the metabolic pathway in root tissues. The protocol shown here is basically applied to other plant Gateway vectors for RNAi.
Gateway（®）系统利用从一个入门载体高通量创建各种表达载体。该技术也应用于RNAi载体，用于植物基因组学的功能分析。迄今为止，已经开发了几种植物Gateway载体并分发给植物科学界。在此，我想介绍为根组织代谢途径功能分析而开发的独特植物Gateway载体。此处所示的方案基本上适用于其他用于RNAi的植物Gateway载体。
