Masclaux Frédéric, Galaud Jean-Philippe
Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland.
Methods Mol Biol. 2011;744:37-55. doi: 10.1007/978-1-61779-123-9_4.
Controlling gene expression during plant development is an efficient method to explore gene function and RNA interference (RNAi) is now considered as a powerful technology for gene functional analysis. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability or plant growth and alternative silencing strategies avoiding these limitations should be preferred. Tissue-specific and inducible promoters, able to control gene expression at spatial and/or temporal level, can be used to circumvent viability problems. In this chapter, after a rapid overview of the inducible promoters currently used for transgenic approaches in plants, we describe a method we have developed to study gene function by heat-inducible RNAi. This system is easy to use and complementary to those based on chemical gene inducer treatments and might be useful for both research and biotechnological applications.
在植物发育过程中控制基因表达是探索基因功能的有效方法，RNA干扰（RNAi）现在被认为是一种用于基因功能分析的强大技术。然而，组成型基因沉默不能用于涉及胚胎活力或植物生长等基本过程的基因，应优先选择避免这些限制的替代沉默策略。能够在空间和/或时间水平上控制基因表达的组织特异性和诱导型启动子可用于规避活力问题。在本章中，在快速概述目前用于植物转基因方法的诱导型启动子之后，我们描述了一种我们开发的通过热诱导RNAi研究基因功能的方法。该系统易于使用，是基于化学基因诱导剂处理的系统的补充，可能对研究和生物技术应用都有用。
