Sirakov Ivo, Peshev Raiko, Christova Lilia
National Diagnostic and Research Veterinary Medical Institute, Prof.Dr G.Pavlov, 15 P. Slaveykov Blvd, Sofia, Bulgaria.
Virus Genes. 2011 Aug;43(1):153-9. doi: 10.1007/s11262-011-0615-7. Epub 2011 Apr 30.
The aim of this study is to investigate the profile of ovine PrP gene by amino acid polymorphism at codons 136, 141, 154, and 171 for determining the genetic predisposition to the Scrapie disease for the tribal sheep and rams, with different numbers and distribution in Bulgaria. Three hundred twenty four animals originating from 41 tribal herds comprising eight breeds were included in the study. DNA was isolated from blood samples specifically amplified by PCR and sequenced. The alignments of codons 136, 141, 154, and 171 were determined. Based on the sequencing, it was established that Bulgarian breeds belong to the second and third risk groups, those with low and moderate risk of Scrapie disease. Establishment of 11 genotypes in Synthetic Population Bulgarian Milk breed reveals it to have the highest risk of the Scrapie disease; moreover, the conducting of the program will be more difficult in comparison with other investigated breeds. Evidence for the internal cross breeding is the presence of the five or six genotypes in the Copper-Red Shoumen, Replian, Karakachan, and Duben Bulgarian native breeds.
本研究的目的是通过对136、141、154和171位密码子处的氨基酸多态性来研究绵羊朊蛋白（PrP）基因的概况，以确定保加利亚不同数量和分布的部落绵羊和公羊对痒病的遗传易感性。本研究纳入了来自41个部落畜群、包含8个品种的324只动物。从经PCR特异性扩增的血样中分离出DNA并进行测序。确定了136、141、154和171位密码子的比对情况。基于测序结果，确定保加利亚品种属于第二和第三风险组，即痒病低风险和中度风险组。合成群体保加利亚乳用品种中确定了11种基因型，表明其患痒病的风险最高；此外，与其他被调查品种相比，实施该计划将更加困难。铜红寿门、雷普良、卡拉卡汗和杜本科保加利亚本土品种中存在五种或六种基因型，这是内部杂交育种的证据。
