绵羊朊病毒蛋白基因中第141和154密码子处的多态性与羊瘙痒病Nor98病例相关。

Polymorphisms at codons 141 and 154 in the ovine prion protein gene are associated with scrapie Nor98 cases.

作者信息

Moum Truls, Olsaker Ingrid, Hopp Petter, Moldal Torfinn, Valheim Mette, Moum Torbjørn, Benestad Sylvie L

机构信息

Norwegian School of Veterinary Science, Department of Production Animal Clinical Sciences, Kyrkjevegen 332-334, N-4325 Sandnes, Norway.

Norwegian School of Veterinary Science, Department of Basic Sciences and Aquatic Medicine, PO Box 8146 Dep., N-0033 Oslo, Norway.

出版信息

J Gen Virol. 2005 Jan;86(Pt 1):231-235. doi: 10.1099/vir.0.80437-0.

DOI:10.1099/vir.0.80437-0

PMID:15604451

Abstract

Until June 2004, thirty-eight scrapie cases with unusual features, designated Nor98, have been diagnosed in Norway. This study investigated the distribution of PrP genotypes among Nor98 cases, their flock-mates and a random sample of Norwegian slaughtered sheep. The PrP genotype distribution of Nor98 cases differed markedly from that of previous cases of classical scrapie. A leucine/phenylalanine polymorphism at codon 141 with hitherto unknown significance to scrapie was strongly associated with Nor98 cases. Twenty of 38 (52.6 %) cases were either homozygous or heterozygous for phenylalanine at codon 141. In contrast, this allele was present in only 10.5 % of the flock-mates and 4.5 % of the random sample of slaughtered sheep. Moreover, the H(154) allele was represented in 24 of 38 (63.2 %) of Nor98 cases, as opposed to 27.0 % of Nor98 flock-mates and 17.0 % of the slaughtered sheep.

摘要

截至2004年6月，挪威已诊断出38例具有异常特征的羊瘙痒病病例，称为Nor98。本研究调查了Nor98病例、其同群羊以及挪威屠宰羊随机样本中PrP基因型的分布情况。Nor98病例的PrP基因型分布与先前经典羊瘙痒病病例的分布明显不同。密码子141处的亮氨酸/苯丙氨酸多态性，此前对羊瘙痒病意义未知，与Nor98病例密切相关。38例病例中有20例（52.6%）在密码子141处为苯丙氨酸纯合子或杂合子。相比之下，该等位基因在同群羊中仅占10.5%，在屠宰羊随机样本中占4.5%。此外，H(154)等位基因在38例Nor98病例中的24例（63.2%）中出现，而在Nor98同群羊中占27.0%，在屠宰羊中占17.0%。