伊朗库尔德斯坦地区马铃薯样本中马铃薯Y病毒（PVy）株系的初步调查。

Preliminary survey of potato virus Y (PVy) strains in potato samples from Kurdistan (Iran).

作者信息

Bahrami-Kamangar S, De Jonghe K, Kamangar S, Maes M, Smagghe G

机构信息

Laboratory of Agrozoology, Faculty of Bioscience Engineering Ghent University, Ghent, Belgium.

出版信息

Commun Agric Appl Biol Sci. 2010;75(4):783-8.

PMID:21534491

Abstract

Potato virus Y (PVY) is the type species in the potyvirus genus of the family potyviridae. This plant pathogenic virus is transmitted through plant sap inoculation by stem and core grafting and by at least 25 aphid species in a non-persistent manner. According to potato specialists in most parts of the world, PVY is currently considered as the most harmful virus in cultivated potatoes. This is also the case for potato production in Iran. In this project we investigated potato leaves that were collected in the Kurdistan province in Iran for the presence of PVY with use of different biochemical/molecular techniques as ELISA, RT-PCR and qPCR. The different PVY strains, including PVY-O, PVY-N, PVYN-TN, PVY-NWi, were determined by using a triplex RT-PCR. In conclusion, the results demonstrated the presence of PVY-NWi strains in the potato leaf samples from Kurdistan (Iran). The data are discussed in relation to prevalence of PVY strains in Iran.

摘要

马铃薯Y病毒（PVY）是马铃薯Y病毒科马铃薯Y病毒属的模式种。这种植物致病病毒通过茎和芯嫁接的植物汁液接种以及至少25种蚜虫以非持久性方式传播。据世界上大多数地区的马铃薯专家称，PVY目前被认为是栽培马铃薯中最有害的病毒。伊朗的马铃薯生产情况也是如此。在本项目中，我们利用酶联免疫吸附测定（ELISA）、逆转录聚合酶链反应（RT-PCR）和定量聚合酶链反应（qPCR）等不同的生化/分子技术，对在伊朗库尔德斯坦省采集的马铃薯叶片进行了PVY检测。通过三重RT-PCR确定了不同的PVY株系，包括PVY-O、PVY-N、PVYN-TN、PVY-NWi。总之，结果表明在来自库尔德斯坦（伊朗）的马铃薯叶片样本中存在PVY-NWi株系。结合伊朗PVY株系的流行情况对数据进行了讨论。