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以色列马铃薯中马铃薯Y病毒群体的特征分析。

Characterization of potato virus Y populations in potato in Israel.

作者信息

Avrahami-Moyal Liat, Tam Yehudit, Sela Noa, Prakash Shanmugam, Meller Harel Yael, Bornstein Menachem, Shulchani Ran, Dar Zion, Gaba Victor

机构信息

Department of Plant Pathology and Weed Science, Agricultural Research Organization-The Volcani Center, Rishon LeZion, 7505101, Israel.

Division for Plant Pests and Diseases, Plant Protection and Inspection Services, Ministry of Agriculture and Rural Development, P.O. Box 78, Bet Dagan, 50250, Israel.

出版信息

Arch Virol. 2019 Jun;164(6):1691-1695. doi: 10.1007/s00705-019-04250-9. Epub 2019 Apr 9.

DOI:10.1007/s00705-019-04250-9
PMID:30968213
Abstract

Potato virus Y (PVY) is the most common virus infecting potato worldwide. We analysed potato tuber PVY infections from the major Israeli growing region in 2014-2017. Isolates were characterized by multiplex PCR according to Chikh-Ali et al. (Plant Disease 97, 1370, 2013), whose primers were not fully compatible with the Israeli isolates. New primers were designed for a multiplex PCR assay to differentiate the Israeli isolates. Three recombinant strains were observed: PVY (72% of the isolates), PVY (24%) and PVY (found only in 2015). The archetypal PVY strain was found only once. The classical PVY strains have recently been displaced by recombinant forms, with PVY dominating. The Israeli isolates appear very similar to those of Europe (the seed tuber source), except for PVY.

摘要

马铃薯Y病毒(PVY)是全球感染马铃薯最常见的病毒。我们分析了2014 - 2017年以色列主要种植区马铃薯块茎的PVY感染情况。根据Chikh - Ali等人(《植物病害》97, 1370, 2013)的方法,通过多重PCR对分离株进行鉴定,但其引物与以色列分离株不完全兼容。设计了新的引物用于多重PCR检测以区分以色列分离株。观察到三种重组株:PVY(占分离株的72%)、PVY(24%)和PVY(仅在2015年发现)。典型的PVY株仅发现过一次。经典的PVY株最近已被重组形式取代,其中PVY占主导地位。除了PVY外,以色列分离株似乎与欧洲(种薯来源地)的分离株非常相似。

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