School of Chemistry, National University of Ireland Galway, Galway, Ireland.
ACS Appl Mater Interfaces. 2011 Jun;3(6):1980-90. doi: 10.1021/am200204j. Epub 2011 May 31.
The use of thermoresponsive surfaces as platforms for cell culture and cell regeneration has been explored over the last couple of decades. Poly-N-isopropylacrylamide (pNIPAm) is a well characterized thermoresponsive polymer which has an aqueous lower critical solution temperature (LCST) in a physiologically useful range, which allows it to reversibly attract (T < 32 °C) and repel water (T > 32 °C). It is this phenomenon that is exploited in temperature-controlled cell harvesting. pNIPAm coatings are generally poorly cell compatible and a number of complex or expensive techniques have been developed in order to overcome this issue. This study seeks to design a simple one-step system whereby commercially sourced pNIPAm is used to achieve similar results. Films were deposited using the operationally simple but rheologically complex spin coating technique. Reversible temperature modulated cell adhesion was achieved using a variety of different cell lines. This system offers a simplistic and cheaper alternative to methods used elsewhere.
在过去的几十年中，人们一直在探索将温敏表面作为细胞培养和细胞再生的平台。聚 N-异丙基丙烯酰胺（pNIPAm）是一种具有良好特性的温敏聚合物，其在生理上有用的范围内具有较低的临界溶液温度（LCST），这使其能够可逆地吸引（T < 32°C）和排斥水（T > 32°C）。正是这种现象在温度控制的细胞收获中得到了利用。pNIPAm 涂层通常与细胞的相容性较差，因此开发了许多复杂或昂贵的技术来克服这个问题。本研究旨在设计一种简单的一步法系统，使用市售的 pNIPAm 来达到类似的效果。通过操作简单但流变复杂的旋涂技术沉积薄膜。使用各种不同的细胞系实现了可逆的温度调节细胞黏附。与其他地方使用的方法相比，该系统提供了一种更简单、更便宜的替代方法。
