Plant Resources Research Inst., Duksung Women's Univ., Seoul 132-714, Korea.
J Food Sci. 2010 Sep;75(7):M412-6. doi: 10.1111/j.1750-3841.2010.01725.x.
This study investigated antibacterial activities of 4 isothiocyanates (3-butenyl, 4-phentenyl, 2-phenylethyl, and benzyl isothiocyanate) against 4 Gram-positive bacteria (Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, and Staphylococcus aureus) and 7 Gram-negative bacteria (Aeromonas hydrophila, Pseudomonas aeruginosa, Salmonella choleaesuis, Salmonella enterica, Serratia marcescens, Shigella sonnei, and Vibrio parahaemolyticus) by an agar disc diffusion assay. Benzyl isothiocyanate (> 90.00 mm inhibition zone diameter at 0.1 μL/mL) and 2-phenylethyl isothiocyanate (58.33 mm at 0.2 μL/mL) showed large inhibition zones especially against B. cereus. Also, 3-butenyl isothiocyanate (21.67 mm at 1.0 μL/mL) and 4-pentenyl isothiocyanate (19.67 mm at 1.0 μL/mL) displayed potent antibacterial activity against A. hydrophila. Benzyl and 2-phenylethyl isothiocyanate indicated higher activity against most of the pathogenic bacteria than 3-butenyl and 4-pentenyl isothiocyanate, and were more effective against Gram-positive bacteria than Gram-negative bacteria.
本研究采用琼脂扩散法测定了 4 种异硫氰酸酯（3-丁烯基、4-苯烯基、2-苯乙基和苄基异硫氰酸酯）对 4 种革兰氏阳性菌（蜡状芽孢杆菌、枯草芽孢杆菌、单核细胞增生李斯特菌和金黄色葡萄球菌）和 7 种革兰氏阴性菌（嗜水气单胞菌、铜绿假单胞菌、鼠伤寒沙门氏菌、肠炎沙门氏菌、粘质沙雷氏菌、宋内志贺氏菌和副溶血性弧菌）的抗菌活性。苄基异硫氰酸酯（在 0.1μL/mL 时抑制圈直径>90.00mm）和 2-苯乙基异硫氰酸酯（在 0.2μL/mL 时 58.33mm）对蜡状芽孢杆菌表现出较大的抑制圈。此外，3-丁烯基异硫氰酸酯（在 1.0μL/mL 时 21.67mm）和 4-戊烯基异硫氰酸酯（在 1.0μL/mL 时 19.67mm）对嗜水气单胞菌表现出较强的抗菌活性。苄基和 2-苯乙基异硫氰酸酯对大多数病原菌的活性均高于 3-丁烯基和 4-戊烯基异硫氰酸酯，对革兰氏阳性菌的活性高于革兰氏阴性菌。
