Vegetable & Fruit Improvement Center, Dept. of Horticultural Science, Texas A&M Univ., College Station, TX 77843, USA.
J Food Sci. 2010 Nov-Dec;75(9):C703-9. doi: 10.1111/j.1750-3841.2010.01824.x. Epub 2010 Oct 7.
Asparagus (Asparagus officinalis L.) spears are rich in bioactive compounds such as protodioscin, a saponin, and rutin, a flavonoid. Protodioscin and rutin are routinely quantified separately, and an approach permitting simultaneous measurement would significantly improve speed of analysis. We have optimized an extraction procedure and modified a method of high-performance liquid chromatography by coupling to an ultraviolet detector to simultaneously analyze protodioscin and rutin in asparagus extracts. An acidic ethanol solvent was more efficient than methanol, acetonitrile, or water in coextraction of protodioscin and rutin. Protodioscin and rutin were detected at 210 nm, with retention times of 12.6 min and 7.9 min, respectively. The method was validated by high linear correlations between 3.13 and 1000.0 μg/mL for protodioscin (r(2)= 0.9999), and between 0.3 and 1087.5 μg/mL for rutin (r(2)= 0.9997). The limit(s) of detection and quantification for protodioscin were 1.6 μg/mL and 3.13 μg/mL, respectively, and for rutin 0.2 μg/mL and 0.3 μg/mL, respectively. White asparagus spears and the crown of the plants were revealed to be rich sources of protodioscin and contained 2.59 to 10.4 mg/g dry weight. Green asparagus spears, particularly the upper portion, were rich in rutin and contained between 1.51 and 7.29 mg/g dry weight.
芦笋（芦笋 officinalis L.）嫩茎富含生物活性化合物，如薯蓣皂苷元，一种皂素，和芦丁，一种类黄酮。薯蓣皂苷元和芦丁通常分别进行定量分析，而一种允许同时测量的方法将显著提高分析速度。我们已经优化了一种提取程序，并通过与紫外检测器耦合来修改高效液相色谱法，以同时分析芦笋提取物中的薯蓣皂苷元和芦丁。酸性乙醇溶剂比甲醇、乙腈或水更有效地共提取薯蓣皂苷元和芦丁。薯蓣皂苷元和芦丁在 210nm 处检测，保留时间分别为 12.6 分钟和 7.9 分钟。该方法通过薯蓣皂苷元在 3.13 至 1000.0μg/mL 之间的高线性相关性（r²=0.9999），以及芦丁在 0.3 至 1087.5μg/mL 之间的高线性相关性（r²=0.9997）进行了验证。薯蓣皂苷元的检测限和定量限分别为 1.6μg/mL 和 3.13μg/mL，芦丁的检测限和定量限分别为 0.2μg/mL 和 0.3μg/mL。白芦笋嫩茎和植物的冠部被发现是薯蓣皂苷元的丰富来源，其含量为 2.59 至 10.4mg/g 干重。绿芦笋嫩茎，特别是上部，富含芦丁，含量在 1.51 至 7.29mg/g 干重之间。
