采用液相色谱/质谱联用（LC/MS）和高效液相色谱（HPLC）法对芦笋嫩茎中原薯蓣皂苷和芦丁进行定量分析。
Quantification of protodioscin and rutin in asparagus shoots by LC/MS and HPLC methods.
作者信息
Wang Mingfu, Tadmor Yaakov, Wu Qing-Li, Chin Chee-Kok, Garrison Stephen A, Simon James E
机构信息
New Use Agriculture and Natural Plant Products Program and Department of Plant Biology and Pathology, Cook College, Rutgers University, and New Jersey Agricultural Experiment Station, New Brunswick, New Jersey 08901, USA.
出版信息
J Agric Food Chem. 2003 Oct 8;51(21):6132-6. doi: 10.1021/jf0344587.
A liquid chromatography/mass spectrometry (LC/MS) method with selected ion monitoring was developed and validated to analyze the contents of protodioscin and rutin in asparagus. The distribution of rutin and protodioscin within the shoots was found to vary by location, with the tissue closest to the rhizome found to be a rich source of protodioscin, at an average level of 0.025% tissue fresh weight in the three tested lines, while the upper youngest shoot tissue contained the highest amount of rutin at levels of 0.03-0.06% tissue fresh weight. The lower portions of the asparagus shoots that are discarded during grading and processing should instead be considered a promising source of a new value-added nutraceutical product.
建立并验证了一种采用选择离子监测的液相色谱/质谱（LC/MS）方法，用于分析芦笋中原薯蓣皂苷和芦丁的含量。结果发现，芦丁和原薯蓣皂苷在嫩茎中的分布因位置而异，最靠近根茎的组织是原薯蓣皂苷的丰富来源，在三个测试品系中，其平均含量为组织鲜重的0.025%，而最上部的幼嫩茎组织中芦丁含量最高，为组织鲜重的0.03 - 0.06%。在分级和加工过程中被丢弃的芦笋嫩茎下部，反而应被视为一种有前景的新型增值营养产品来源。
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