State Key Lab. of Food Science & Technology and School of Food Science and Technology, Jiangnan Univ., Nr 1800, Lihu Rd., Wuxi 214122, China.
J Food Sci. 2011 Jan-Feb;76(1):C127-32. doi: 10.1111/j.1750-3841.2010.01916.x. Epub 2010 Nov 29.
Effects of pulsed electric field (PEF) treatments on the volatile profiles of milk were studied and compared with pasteurized treatment of high temperature short time (HTST) (75 °C, 15 s). Volatile compounds were extracted by solid-phase micro-extraction (SPME) and identified by gas chromatography/mass spectrometry (GC-MS) and gas chromatography-olfactometry (GC-O). A total of 37 volatile compounds were determined by GC-MS, and 19 volatile compounds were considered to be major contributors to the characteristic flavor of milk samples. PEF treatment resulted in an increase in aldehydes. Milk treated with PEF at 30 kV/cm showed the highest content of pentanal, hexanal, and nonanal, while heptanal and decanal contents were lower than in pasteurized milk, but higher than in raw milk. All the methyl ketones detected in PEF milk were lower than in pasteurized milk. No significant differences in acids (acetic acid, butanoic acid, hexanoic acid, octanoic acid, and decanoic acid), lactones, and alcohols were observed between pasteurized and PEF-treated samples; however, 2(5H)-furanone was only detected in PEF-treated milk. Although GC-MS results showed that there were some volatile differences between pasteurized and PEF-treated milk, GC-O data showed no significant difference between the 2 samples.
研究了脉冲电场(PEF)处理对牛奶挥发性成分的影响,并将其与高温短时间巴氏杀菌处理(75°C,15 s)进行了比较。采用固相微萃取(SPME)提取挥发性化合物,并用气相色谱/质谱联用(GC-MS)和气相色谱-嗅闻(GC-O)进行鉴定。通过 GC-MS 共鉴定出 37 种挥发性化合物,其中 19 种被认为是牛奶样品特征风味的主要贡献者。PEF 处理会导致醛含量增加。在 30 kV/cm 的 PEF 处理下,牛奶中戊醛、己醛和壬醛的含量最高,而庚醛和壬醛的含量低于巴氏杀菌奶,但高于生奶。在 PEF 牛奶中检测到的所有甲基酮都低于巴氏杀菌奶。巴氏杀菌和 PEF 处理的样品之间在酸(乙酸、丁酸、己酸、辛酸和癸酸)、内酯和醇方面没有观察到显著差异;然而,2(5H)-呋喃酮仅在 PEF 处理的牛奶中被检测到。尽管 GC-MS 结果表明巴氏杀菌和 PEF 处理的牛奶之间存在一些挥发性差异,但 GC-O 数据显示这两种样品之间没有显著差异。