Nakahata N, Abe M T, Matsuoka I, Nakanishi H
Department of Pharmacology, Fukushima Medical College, Japan.
FEBS Lett. 1990 Jan 15;260(1):91-4. doi: 10.1016/0014-5793(90)80074-s.
Mastoparan inhibited [3H]inositol phosphate accumulation induced by carbachol as well as cyclic AMP accumulation induced by isoproterenol in 1321N1 human astrocytoma cells. Mastoparan inhibited GTP gamma S-induced, but not Ca2(+)-induced, [3H]inositol phosphate accumulation in membrane preparations with an IC50 of approximately 10 microM. The inhibitory effect of mastoparan on carbachol-induced [3H]inositol phosphate accumulation was resistant to pertussis toxin (IAP) treatment in intact cells. These results suggest that mastoparan inhibits phospholipase C in human astrocytoma cells via a GTP binding protein, which is not a substrate for IAP.
马斯托帕兰抑制1321N1人星形细胞瘤细胞中由卡巴胆碱诱导的[3H]肌醇磷酸积累以及由异丙肾上腺素诱导的环磷酸腺苷积累。马斯托帕兰抑制膜制剂中GTPγS诱导的而非Ca2 +诱导的[3H]肌醇磷酸积累，IC50约为10微摩尔。马斯托帕兰对卡巴胆碱诱导的[3H]肌醇磷酸积累的抑制作用在完整细胞中对百日咳毒素（IAP）处理具有抗性。这些结果表明，马斯托帕兰通过一种GTP结合蛋白抑制人星形细胞瘤细胞中的磷脂酶C，该GTP结合蛋白不是IAP的底物。
