Inst of Biochemistry and Biotechnology, Chung Shan Medical Univ, No 110, Section 1, Chien-kauo N Rd Taichung, Taiwan, ROC.
J Food Sci. 2011 Mar;76(2):T65-72. doi: 10.1111/j.1750-3841.2010.02033.x.
Excessive lipid accumulation within liver has been proposed to cause obesity, hyperlipidemia, diabetes, and fatty liver disease. Rutin, a common dietary flavonoid that is consumed in fruits, vegetables, and plant-derived beverages, has various biological functions, including antioxidant, anti-inflammatory, and anticancer effects. However, a hypolipidemic effect of rutin on fatty liver disease has not been reported. In this study, we examined the effect of rutin on reducing lipid accumulation in hepatic cells. Hepatocytes were treated with oleic acid (OA) containing with or without rutin to observe the lipid accumulation by Nile red stain. The result showed rutin suppressed OA-induced lipid accumulation and increased adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) activity in hepatocytes. The expression of critical molecule involved in lipid synthesis, sterol regulatory element binding proteins-1 (SREBP-1), was attenuated in rutin-treated cells. Moreover, long-term incubation of rutin inhibited the transcriptions of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (HMGCR), glycerol-3-phosphate acyltransferase (GPAT), fatty acid synthase (FAS), and acetyl-coenzyme carboxylase (ACC). Besides, we also found out the antioxidative effect of rutin by increasing the expression of peroxisome proliferator-activated receptor (PPAR)-α and antioxidative enzymes. Taken together, our findings suggest rutin could attenuate lipid accumulation by decreasing lipogenesis and oxidative stress in hepatocyte.
过量的脂质在肝脏中积累被认为会导致肥胖、高血脂、糖尿病和脂肪肝疾病。芦丁是一种常见的饮食类黄酮，存在于水果、蔬菜和植物源性饮料中，具有多种生物学功能，包括抗氧化、抗炎和抗癌作用。然而，芦丁对脂肪肝疾病的降血脂作用尚未被报道。在这项研究中，我们研究了芦丁对减少肝细胞脂质积累的作用。用含有或不含有芦丁的油酸（OA）处理肝细胞，通过尼罗红染色观察脂质积累情况。结果表明，芦丁抑制 OA 诱导的脂质积累，并增加肝细胞中的腺苷 5'-单磷酸（AMP）激活蛋白激酶（AMPK）活性。参与脂质合成的关键分子固醇调节元件结合蛋白-1（SREBP-1）的表达在芦丁处理的细胞中减弱。此外，芦丁的长期孵育抑制了 3-羟-3-甲基戊二酰辅酶 A（HMG-CoA）还原酶（HMGCR）、甘油-3-磷酸酰基转移酶（GPAT）、脂肪酸合成酶（FAS）和乙酰辅酶 A 羧化酶（ACC）的转录。此外，我们还发现芦丁通过增加过氧化物酶体增殖物激活受体（PPAR）-α和抗氧化酶的表达来发挥抗氧化作用。综上所述，我们的研究结果表明，芦丁可以通过减少肝实质细胞的脂肪生成和氧化应激来减轻脂质积累。
