Gu Min Ji, Ahn Yejin, Lee Yu Ra, Yoo Guijae, Kim Yoonsook, Choi Inwook, Ha Sang Keun, Kim Donghwan
Korea Food Research Institute, Wanju-gun 55365, Republic of Korea.
Division of Food Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea.
Nutrients. 2024 Nov 30;16(23):4165. doi: 10.3390/nu16234165.
Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease. In recent times, the term NAFLD has been modified to metabolic dysfunction-associated steatotic liver disease (MASLD), reflecting its comprehensive scope encompassing a range of metabolic abnormalities. L. (CS) is a traditional medicine, although the preventive mechanism of CS extracts remains unclear.
This study evaluated the preventive effects of CS in high-fat diet (HFD)-induced MASLD mice by oral administration of 100 or 200 mg/kg/day of CS extracts for 12 weeks.
The major CS extract compounds were chlorogenic acid, caffeic acid, rutin, and isoquercetin. The administration of CS extract suppressed HFD-induced weight gain, liver weight, and the liver/body weight ratio. It improved the mice's serum biological profiles and suppressed HFD-induced lipid droplet and lipid accumulation by inhibiting lipid accumulation-related gene expression in the liver. It modulated HFD-induced Ampk-Srebp1c pathways and suppressed HFD-induced NF-κB pathway activation in the liver. It regulated inflammation and the AMPK alpha signaling pathway in HFD-fed mice by reducing the accumulation of specific amino acids, leading to the amelioration of fatty liver.
The CS extract prevents HFD-induced MASLD and may help prevent or treat MASLD.
非酒精性脂肪性肝病（NAFLD）是最常见的慢性肝病。近年来，NAFLD这一术语已被修改为代谢功能障碍相关脂肪性肝病（MASLD），反映了其涵盖一系列代谢异常的全面范畴。杜仲（CS）是一种传统药物，但其提取物的预防机制尚不清楚。
本研究通过对高脂饮食（HFD）诱导的MASLD小鼠口服给予100或200 mg/kg/天的CS提取物，持续12周，评估CS的预防作用。
CS提取物的主要化合物为绿原酸、咖啡酸、芦丁和异槲皮苷。给予CS提取物可抑制HFD诱导的体重增加、肝脏重量及肝/体重比。它改善了小鼠的血清生物学指标，并通过抑制肝脏中脂质积累相关基因的表达，抑制了HFD诱导的脂滴和脂质积累。它调节了HFD诱导的Ampk-Srebp1c信号通路，并抑制了HFD诱导的肝脏中NF-κB信号通路的激活。它通过减少特定氨基酸的积累来调节HFD喂养小鼠的炎症和AMPKα信号通路，从而改善脂肪肝。
CS提取物可预防HFD诱导的MASLD，可能有助于预防或治疗MASLD。
