Baker M E
Department of Medicine, University of California, San Diego, La Jolla 92093.
FASEB J. 1990 Feb 1;4(2):222-6. doi: 10.1096/fasebj.4.2.2153594.
A computer-based comparison of the amino acid sequences of human placental 17 beta-hydroxysteroid dehydrogenase and Streptomyces coelicolor actIII protein, which is important in the synthesis of the antibiotic actinorhodin, gives an alignment score 12.15 standard deviations higher than that of 1000 comparisons of randomized sequences of these proteins. The probability of getting this score by chance is 3 x 10(-34). Comparison of actIII protein with Drosophila melanogaster alcohol dehydrogenase yields a score of 10.3 standard deviations (P = 3.5 x 10(-25)). Based on these similarities, we propose that 17 beta-hydroxysteroid dehydrogenase, actIII protein, and Drosophila alcohol dehydrogenase are derived from a common ancestor.
通过计算机对人胎盘17β-羟基类固醇脱氢酶与天蓝色链霉菌actIII蛋白的氨基酸序列进行比较，该蛋白在抗生素放线紫红素的合成中起重要作用，其比对得分比这两种蛋白随机序列的1000次比较结果高12.15个标准差。偶然获得该得分的概率为3×10⁻³⁴。将actIII蛋白与黑腹果蝇乙醇脱氢酶进行比较，得分是10.3个标准差（P = 3.5×10⁻²⁵）。基于这些相似性，我们提出17β-羟基类固醇脱氢酶、actIII蛋白和果蝇乙醇脱氢酶源自共同的祖先。
