Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Liouying, Taiwan; School of Dentistry, National Yang-Ming University, Taipei, Taiwan.
Oral Oncol. 2011 Jun;47(6):452-8. doi: 10.1016/j.oraloncology.2011.03.025. Epub 2011 May 4.
Oral cancer has emerged as one of the fastest growing malignancies in Taiwan. However, biomarkers that reliably predict clinical outcomes have yet to be identified. This study was aimed to identify tumor-associated proteins that could be prognostic biomarkers for oral cancer. We compared the protein expression between oral squamous cell carcinoma (OSCC) tissues and adjacent non-cancerous matched tissues (NCMTs) by proteomics. We found that Rho GDP-dissociation inhibitor alpha (RhoGDIα) was differentially expressed in frozen cancerous samples and OSCC cell lines but not in NCMTs. Furthermore, our results indicated that RhoGDIα was selectively upregulated in 78 OSCC tissue sections (p<0.001), and this high expression was significantly correlated with increased tumor size (p<0.05) and poor overall survival (p<0.01). There was a trend that RhoGDIα expression was localized in the cytoplasm of cancer cells but was localized in the plasma membrane of NCMTs. Finally, expression of RhoGDIα was validated to be an independent prognostic indicator for overall survival (p<0.01). These results have identified a novel biomarker that may be useful for prediction of poor prognosis in OSCC patients.
口腔癌已成为台湾增长最快的恶性肿瘤之一。然而，仍未发现可靠预测临床结果的生物标志物。本研究旨在确定肿瘤相关蛋白，作为口腔癌的预后生物标志物。我们通过蛋白质组学比较了口腔鳞状细胞癌（OSCC）组织和相邻非癌性配对组织（NCMTs）之间的蛋白表达。我们发现 Rho GDP 解离抑制剂 alpha（RhoGDIα）在冷冻癌组织样本和 OSCC 细胞系中差异表达，但在 NCMTs 中没有。此外，我们的结果表明，RhoGDIα在 78 个 OSCC 组织切片中选择性上调（p<0.001），并且这种高表达与肿瘤体积增大（p<0.05）和总生存率降低（p<0.01）显著相关。有趋势表明，RhoGDIα在癌细胞的细胞质中表达，但在 NCMTs 的质膜中表达。最后，RhoGDIα 的表达被验证为总生存率的独立预后指标（p<0.01）。这些结果确定了一种新的生物标志物，可能有助于预测 OSCC 患者的不良预后。
