使用 CaCO₃ 涂覆的气球微球在液-液界面生物反应器（L-L IBR）中用真菌脂肪酶高效水解乙酸酯。
Efficient hydrolytic reaction of an acetate ester with fungal lipase in a liquid-liquid interface bioreactor (L-L IBR) using CaCO₃-coated ballooned microsphere.
机构信息
Genome Biotechnology Laboratory, Kanazawa Institute of Technology, 3-1 Yatsukaho, Hakusan, Ishikawa 924-0838, Japan.
出版信息
J Biosci Bioeng. 2011 Aug;112(2):151-3. doi: 10.1016/j.jbiosc.2011.04.001. Epub 2011 May 1.
PMID:21536495
Abstract
In a liquid-liquid interface bioreactor using a CaCO₃-coated ballooned microsphere, 2-ethylhexyl acetate was efficiently hydrolyzed to 2-ethyl-1-hexanol with Absidia coerulea NBRC 4423 compared with using talc-coated or non-coated ballooned microsphere. It was assumed that CaCO₃ brought about stabilization of lipase by Ca²⁺ and maintenance of medium pH.
摘要
在使用 CaCO3 涂层的气球微球的液-液界面生物反应器中，与使用滑石粉涂层或无涂层的气球微球相比，青霉菌 NBRC 4423 可有效地将 2-乙基己基乙酸酯水解为 2-乙基-1-己醇。据推测，CaCO3 通过 Ca²⁺ 稳定脂肪酶并维持介质 pH 值。
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