Department of Developmental and Molecular Biology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA.
J Biol Chem. 2011 Jun 17;286(24):21915-26. doi: 10.1074/jbc.M110.209460. Epub 2011 May 2.
The Golgi apparatus is a network of polarized cisternae localized to the perinuclear region in mammalian cells. It undergoes extensive vesiculation at the onset of mitosis and its reassembly requires factors that are in part segregated via the mitotic spindle. Here we show that unlike typical Golgi markers, the Golgi-protein p115 partitioned with the spindle poles throughout mitosis. An armadillo-fold in its N terminus mediated a novel interaction between p115 and γ-tubulin and functioned in its centrosomal targeting. Both the N- and C-terminal regions of p115 were required to maintain Golgi structure. Strikingly, p115 was essential for mitotic spindle function and the resolution of the cytokinetic bridge because its depletion resulted in spindle collapse, chromosome missegregation, and failed cytokinesis. We demonstrate that p115 plays a critical role in mitosis progression, implicating it as the only known golgin to regulate both mitosis and apoptosis.
高尔基器是一种位于哺乳动物细胞核周区域的极化潴泡网络。在有丝分裂开始时，它会经历广泛的泡状化，其重新组装需要部分通过有丝分裂纺锤体进行分隔的因子。在这里，我们表明与典型的高尔基体标记物不同，高尔基蛋白 p115 在整个有丝分裂过程中与纺锤体极一起分配。其 N 端的一个卷曲螺旋结构介导了 p115 和 γ-微管蛋白之间的新相互作用，并在其中心体靶向中发挥作用。p115 的 N 端和 C 端区域都需要维持高尔基体结构。引人注目的是，p115 对于有丝分裂纺锤体功能和胞质分裂桥的解决至关重要，因为其耗竭导致纺锤体崩溃、染色体错误分离和胞质分裂失败。我们证明 p115 在有丝分裂进程中起着关键作用，表明它是唯一已知的调节有丝分裂和细胞凋亡的高尔基体蛋白。
