高压氧疗法对保留脾下极的大鼠脾次全切除术的影响。

Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole.

作者信息

Paulo Marcela Souza Lima, Paulo Isabel Cristina Andreatta Lemos, Nunes Tarcizo Afonso, Silva Alcino Lázaro da, Cintra Luiz Cálice, Paulo Danilo Nagib Salomão

机构信息

EMESCAM, Vitoria-ES, Brazil.

出版信息

Acta Cir Bras. 2011 Jun;26(3):156-64. doi: 10.1590/s0102-86502011000300002.

DOI:10.1590/s0102-86502011000300002

PMID:21537516

Abstract

PURPOSE

To evaluate the effect of hyperbaric oxygen therapy on the survival and weight of rats submitted to subtotal splenectomy and on the viability and growth of the inferior pole.

METHODS

Forty Wistar rats underwent subtotal splenectomy with preservation of the inferior pole and distributed into two groups: Group A (n=20) - not treated with hyperbaric oxygen, Group B (n=20) – treated with hyperbaric oxygen. These groups were divided into two subgroups of 10 animals each, according to the time of euthanasia, 15th and 45th days. The survival and weight of the animals were recorded. The inferior pole was measured, weighed and morphologically analyzed.

RESULTS

All animals survived. The weight of the animals increased in all subgroups, but decreased on the 10th day in the subgroups treated with hyperbaric oxygen (p<0.001). The viability of the inferior pole was more evident in animals treated on the 15th day, but did not differ on the 45th day. The growth of the inferior pole has not occurred on the 15th day but on the 45th day after surgery in untreated animals (p<0.01) and treated animals (p<0.05). Vascular and cellular increase in treated animals was significantly higher than in untreated ones.

CONCLUSION

Hyperbaric oxygen therapy did not affect the survival of animals but reduced their weight. It improved the viability of the inferior splenic pole, but did not interfere with their growth.

摘要

目的

评估高压氧疗法对接受脾次全切除术大鼠的存活及体重，以及对脾下极活力和生长的影响。

方法

40只Wistar大鼠接受脾次全切除术并保留脾下极，分为两组：A组（n = 20）——未接受高压氧治疗，B组（n = 20）——接受高压氧治疗。根据安乐死时间，每组再分为两个亚组，每组10只动物，分别在第15天和第45天安乐死。记录动物的存活情况和体重。对脾下极进行测量、称重并进行形态学分析。

结果

所有动物均存活。所有亚组动物体重均增加，但接受高压氧治疗的亚组在第10天时体重下降（p < 0.001）。脾下极的活力在第15天接受治疗的动物中更明显，但在第45天时无差异。未治疗动物和治疗动物在术后第15天脾下极未生长，但在第45天生长（未治疗动物p < 0.01，治疗动物p < 0.05）。治疗动物的血管和细胞增加明显高于未治疗动物。