Borjaili Andréa Saade Daher, Nunes Giseli Celestino, Silveira Julia Belizário, Dall'Orto Flávia Heiderich, Lorenzoni Gabriel Souza, Borjaili Matheus Eduardo Daher, Campagnaro Bianca Prandi, Lima Maressa Cristiane Malini de, Paulo Marcela Souza Lima, Paulo Danilo Nagib Salomão, Nunes Tarcizo Afonso
Fellow PhD degree, Postgraduate Program in Surgical and Ophthalmological Applied Sciences, Medical School, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte-MG, Brazil. Scientific and intellectual contributions to the study, analysis and interpretation of data, manuscript preparation and writing.
Graduate student, School of Sciences, Santa Casa de Misericórdia de Vitória (EMESCAM), Vitoria-ES, Brazil. Acquisition of data, technical procedures.
Acta Cir Bras. 2020 Nov 23;35(10):e202001003. doi: 10.1590/s0102-865020200100000003. eCollection 2020.
Comparing survival rates of rats subjected to spleen procedures after fecal peritonitis induction. Assessing changes in TCD4 and CD8 lymphocyte rates before and after the procedures. Correlating animal survival with CD4 and CD8 lymphocytes.
Thirty male Wistar rats were distributed into 3 groups of ten: spleen manipulation (SM); total splenectomy (TS); subtotal splenectomy with preservation of the inferior pole (IP). Rats were subjected to surgical procedure depending on the group. Seven days after surgery they underwent induction of peritonitis and survival time was recorded. All animals were subjected to two blood collections: before surgery and 70 days after it for TCD4/TCD8 lymphocyte counting.
Mean survival time was longer in the IP and SM groups and shorter in the TS group; there was significant difference between them. The comparison of the median number of CD4 did not present changes, whereas the comparison of the median number of CD8 decreased in the SM and IP groups. The correlation between the median number of TCD4 and TCD8 lymphocytes and the animals' survival was not significant.
The maintenance of splenic tissue contributed to increase the survival of rats and there was a change in the number of TCD8 lymphocytes.
比较诱导粪性腹膜炎后接受脾脏手术的大鼠的存活率。评估手术前后TCD4和CD8淋巴细胞比率的变化。将动物存活率与CD4和CD8淋巴细胞进行关联分析。
30只雄性Wistar大鼠被分为3组，每组10只：脾脏操作（SM）组；全脾切除术（TS）组；保留脾下极的次全脾切除术（IP）组。根据分组对大鼠进行手术操作。术后7天诱导腹膜炎，并记录存活时间。所有动物在手术前和手术后70天进行两次采血，以计数TCD4/TCD8淋巴细胞。
IP组和SM组的平均存活时间较长，TS组较短；三组之间存在显著差异。CD4中位数的比较没有变化，而SM组和IP组中CD8中位数的比较有所下降。TCD4和TCD8淋巴细胞中位数与动物存活率之间的相关性不显著。
脾脏组织的保留有助于提高大鼠的存活率，并且TCD8淋巴细胞数量发生了变化。
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