Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, Tokushima City, Japan.
Dev Dyn. 2011 Jun;240(6):1440-53. doi: 10.1002/dvdy.22647. Epub 2011 Apr 28.
In the cricket Gryllus bimaculatus, missing distal parts of amputated legs are regenerated from blastemas based on positional information. The Dachsous/Fat (Ds/Ft) signaling pathway regulates blastema cell proliferation and positional information along the longitudinal axis during leg regeneration. Herein, we show that the Gryllus homologue of Lowfat (Gb'Lft), which modulates Ds/Ft signaling in Drosophila, is involved in leg regeneration. Gb'lft is expressed in regenerating legs, and RNAi against Gb'lft (Gb'lft(RNAi)) suppressed blastema cell hyperproliferation caused by Gb'ft(RNAi) or Gb'ds(RNAi) but enhanced that caused by Gb'kibra(RNAi) or Gb'warts(RNAi). In Gb'lft(RNAi) nymphs, missing parts of amputated legs were regenerated, but the length of the regenerated legs was shortened depending on the position of the amputation. Both normal and reversed intercalary regeneration occurred in Gb'lft(RNAi) nymphs, suggesting that Gb'Lft is involved in blastema cell proliferation and longitudinal leg regeneration under the Ds/Ft signaling pathway, but it is not required for intercalary regeneration.
在蟋蟀 Gryllus bimaculatus 中，截肢后缺失的远端部分是从芽基根据位置信息再生的。Dachsous/Fat (Ds/Ft) 信号通路在腿再生过程中调节芽基细胞的增殖和沿纵轴的位置信息。在此，我们表明，调节果蝇中 Ds/Ft 信号的 Lowfat (Gb'Lft) 的蟋蟀同源物参与了腿再生。Gb'lft 在再生的腿中表达，针对 Gb'lft 的 RNAi（Gb'lft(RNAi)）抑制了由 Gb'ft(RNAi) 或 Gb'ds(RNAi) 引起的芽基细胞过度增殖，但增强了由 Gb'kibra(RNAi) 或 Gb'warts(RNAi) 引起的过度增殖。在 Gb'lft(RNAi) 若虫中，缺失的截肢腿部分得到了再生，但再生腿的长度根据截肢的位置而缩短。在 Gb'lft(RNAi) 若虫中，正常和反向的间插再生都发生了，这表明 Gb'Lft 参与了芽基细胞增殖和 Ds/Ft 信号通路下的纵向腿再生，但它不是间插再生所必需的。
