Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1 Shikata-cho, Kita-ku, Okayama city, Okayama, 700-8530, Japan.
Development. 2013 Mar;140(5):959-64. doi: 10.1242/dev.084590. Epub 2013 Jan 23.
In the cricket Gryllus bimaculatus, missing distal parts of the amputated leg are regenerated from the blastema, a population of dedifferentiated proliferating cells that forms at the distal tip of the leg stump. To identify molecules involved in blastema formation, comparative transcriptome analysis was performed between regenerating and normal unamputated legs. Components of JAK/STAT signalling were upregulated more than twofold in regenerating legs. To verify their involvement, Gryllus homologues of the interleukin receptor Domeless (Gb'dome), the Janus kinase Hopscotch (Gb'hop) and the transcription factor STAT (Gb'Stat) were cloned, and RNAi was performed against these genes. Gb'dome(RNAi), Gb'hop(RNAi) and Gb'Stat(RNAi) crickets showed defects in leg regeneration. Blastema expression of Gb'cyclinE was decreased in the Gb'Stat(RNAi) cricket compared with that in the control. Hyperproliferation of blastema cells caused by Gb'fat(RNAi) or Gb'warts(RNAi) was suppressed by RNAi against Gb'Stat. The results suggest that JAK/STAT signalling regulates blastema cell proliferation during leg regeneration.
在蟋蟀 Gryllus bimaculatus 中，截肢后缺失的腿部远端部分是从芽基再生的，芽基是一种分化增殖细胞的群体，形成于腿部残端的远端。为了鉴定参与芽基形成的分子，对再生和正常未截肢的腿部进行了比较转录组分析。JAK/STAT 信号通路的组成部分在再生的腿部中上调了两倍以上。为了验证它们的参与，克隆了蟋蟀的白细胞介素受体无顶（Gb'dome）、Janus 激酶 Hopscotch（Gb'hop）和转录因子 STAT（Gb'Stat）的同源物，并针对这些基因进行了 RNAi 实验。Gb'dome（RNAi）、Gb'hop（RNAi）和 Gb'Stat（RNAi）蟋蟀在腿部再生方面表现出缺陷。与对照相比，Gb'Stat（RNAi）蟋蟀的芽基 Gb'cyclinE 表达减少。Gb'fat（RNAi）或 Gb'warts（RNAi）引起的芽基细胞过度增殖，通过针对 Gb'Stat 的 RNAi 得到了抑制。结果表明，JAK/STAT 信号通路在腿部再生过程中调节芽基细胞的增殖。
