Institute of Biotechnology, University of Lausanne, Switzerland.
J Biotechnol. 2011 Jun 10;154(1):11-20. doi: 10.1016/j.jbiotec.2011.04.004. Epub 2011 Apr 22.
Matrix attachment regions are DNA sequences found throughout eukaryotic genomes that are believed to define boundaries interfacing heterochromatin and euchromatin domains, thereby acting as epigenetic regulators. When included in expression vectors, MARs can improve and sustain transgene expression, and a search for more potent novel elements is therefore actively pursued to further improve recombinant protein production. Here we describe the isolation of new MARs from the mouse genome using a modified in silico analysis. One of these MARs was found to be a powerful activator of transgene expression in stable transfections. Interestingly, this MAR also increased GFP and/or immunoglobulin expression from some but not all expression vectors in transient transfections. This effect was attributed to the presence or absence of elements on the vector backbone, providing an explanation for earlier discrepancies as to the ability of this class of elements to affect transgene expression under such conditions.
基质附着区是在真核基因组中发现的 DNA 序列，被认为定义了异染色质和常染色质域的界面边界，从而作为表观遗传调节剂发挥作用。当包含在表达载体中时，MAR 可以改善和维持转基因的表达，因此积极寻找更有效的新型元件，以进一步提高重组蛋白的生产。在这里，我们使用改良的计算机分析从小鼠基因组中分离新的 MAR。其中一个 MAR 被发现是稳定转染中转基因表达的有力激活子。有趣的是，该 MAR 还增加了 GFP 和/或免疫球蛋白的表达，但不是所有瞬时转染的表达载体都有表达。这种效应归因于载体骨架上存在或不存在元件，这就解释了为什么在这种情况下，这一类元件影响转基因表达的能力存在差异。
