Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama, Japan.
Analyst. 2011 Jun 21;136(12):2446-9. doi: 10.1039/c1an15130d. Epub 2011 May 3.
In this study, non-engineered RNAs were detected in living cells using bioluminescence. Two types of probe were utilized: a peptide inserted RLuc (PI-RLuc) probe and a split-RNA probe. Incorporation of the PI-RLuc and split-RNA probes enabled the direct detection of RNA introduced into living cells.
在这项研究中，使用生物发光在活细胞中检测到非工程化的 RNA。使用了两种类型的探针：插入肽的 RLuc（PI-RLuc）探针和分裂 RNA 探针。PI-RLuc 探针和分裂 RNA 探针的掺入使得能够直接检测引入活细胞的 RNA。
