用于活体成像的红移型海肾荧光素酶变体。
Red-shifted Renilla reniformis luciferase variants for imaging in living subjects.
作者信息
Loening Andreas Markus, Wu Anna M, Gambhir Sanjiv Sam
机构信息
Molecular Imaging Program at Stanford, Departments of Radiology and Bioengineering, Bio-X Program, Stanford University, Stanford, California 94305, USA.
出版信息
Nat Methods. 2007 Aug;4(8):641-3. doi: 10.1038/nmeth1070. Epub 2007 Jul 8.
The use of R. reniformis luciferase (RLuc) as a reporter gene in small-animal imaging has been hampered by its 481 nm peaked emission spectrum, as blue wavelengths are strongly attenuated in biological tissues. To overcome this, we generated variants of RLuc with bathochromic (red) shifts of up to 66 nm (547 nm peak) that also had greater stability and higher light emission than native RLuc.
肾形丽蚌萤光素酶（RLuc）作为一种报告基因用于小动物成像时，因其发射光谱峰值在481nm，而蓝色波长在生物组织中会被强烈衰减，故而受到限制。为克服这一问题，我们生成了肾形丽蚌萤光素酶的变体，其红移（向红色方向移动）可达66nm（峰值为547nm），并且与天然肾形丽蚌萤光素酶相比，具有更高的稳定性和发光强度。
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