Delacadena M, Fernandez J, Decarlos A, Martinezzorzano V, Gilmartin E, Rodriguezberrocal F
Int J Oncol. 1996 Oct;9(4):747-54. doi: 10.3892/ijo.9.4.747.
Colorectal cancer remains a major medical problem and survival of the patients is directly correlated to the possibility of metastases occurrence. We searched for biochemical differences between colorectal adenocarcinomas and their precursor normal cells with the aim of complementing histological methods for the classification and prognosis of human colorectal tumours. Increased fucosylation is one of the most common phenomena associated with malignant transformation, invasion and metastases. It might be expected that the enzyme alpha-L-fucosidase (alpha-L-fucosidase fucohydrolase E.C.3.2.1.51), involved in the breakdown of fucose-containing glycoproteins and glycolipids, would play an important role in the maintenance of the fucose content of aberrant fucosylated glycoconjugates. Statistical evaluation of 110 patients with colorectal adenocarcinoma has shown that there is a significant decrease of alpha-L-fucosidase activity in the malignant tissue compared with the healthy colonic mucosa of the same patient. Characterisation studies have shown that there are remarkable similarities in the physicochemical and kinetic properties of the enzyme in both tissues. Using an immunodetection assay we conclude that the observed decrease of alpha-L-fucosidase activity is due to a significant decrease of alpha-L-fucosidase protein in colorectal tumours. Our results further demonstrate that the alpha-L-fucosidase content (either as enzymatic activity or as enzymatic protein) is lower in primary tumours at advanced stages (Dukes' B and C) than in primaries at early stages (Dukes' A).
结直肠癌仍然是一个主要的医学问题，患者的生存率与转移发生的可能性直接相关。我们寻找结直肠腺癌与其前体正常细胞之间的生化差异，旨在补充用于人类结直肠肿瘤分类和预后的组织学方法。岩藻糖基化增加是与恶性转化、侵袭和转移相关的最常见现象之一。可以预期，参与含岩藻糖糖蛋白和糖脂分解的α-L-岩藻糖苷酶（α-L-fucosidase fucohydrolase E.C.3.2.1.51）在维持异常岩藻糖基化糖缀合物的岩藻糖含量方面将发挥重要作用。对110例结直肠腺癌患者的统计评估表明，与同一患者的健康结肠黏膜相比，恶性组织中α-L-岩藻糖苷酶活性显著降低。特性研究表明，两种组织中该酶的物理化学和动力学特性存在显著相似性。使用免疫检测分析，我们得出结论，观察到的α-L-岩藻糖苷酶活性降低是由于结直肠肿瘤中α-L-岩藻糖苷酶蛋白显著减少所致。我们的结果进一步表明，晚期（杜克B期和C期）原发性肿瘤中的α-L-岩藻糖苷酶含量（以酶活性或酶蛋白形式）低于早期（杜克A期）原发性肿瘤。
