血清α-L-岩藻糖苷酶活性在结直肠癌诊断中的价值。

Value of the serum alpha-L-fucosidase activity in the diagnosis of colorectal cancer.

作者信息

Ayude D, Fernández-Rodríguez J, Rodríguez-Berrocal F J, Martínez-Zorzano V S, de Carlos A, Gil E, Páez de La Cadena M

机构信息

Department of Biochemistry, Genetics and Immunology, University of Vigo, Spain.

出版信息

Oncology. 2000 Nov;59(4):310-6. doi: 10.1159/000012188.

DOI:10.1159/000012188

PMID:11096343

Abstract

OBJECTIVES

The purpose of this study was to assess the value of the serum levels of alpha-L-fucosidase activity in the diagnosis of patients with colorectal cancer.

METHODS

Using a fluorometric method we analyzed the alpha-L-fucosidase activity in preoperative sera from 137 colorectal cancer patients and in sera from 232 donors.

RESULTS

The enzymatic activity of alpha-L-fucosidase was significantly lower (p <0.001) in patients (4.8+/-3.09 U/ml) than in donors (10.5+/-5.46 U/ml). Using the ROC curve, the ideal cut-off for the diagnostic value of alpha-L-fucosidase activity was determined to be 5.6 U/ml. The diagnostic efficiency for colorectal cancer of alpha-L-fucosidase activity was higher than that observed for carcinoembryonic antigen (cut-off 5.0 ng/ml), especially for tumors at an early stage.

CONCLUSIONS

Our results suggest that preoperative serum alpha-L-fucosidase activity may be used as a cheap and easy complementary test, in addition to standard clinical procedures routinely used for the diagnosis of colorectal cancer.

摘要

目的

本研究旨在评估血清α-L-岩藻糖苷酶活性水平在结直肠癌患者诊断中的价值。

方法

我们采用荧光法分析了137例结直肠癌患者术前血清及232例献血者血清中的α-L-岩藻糖苷酶活性。

结果

患者（4.8±3.09 U/ml）的α-L-岩藻糖苷酶酶活性显著低于献血者（10.5±5.46 U/ml）（p<0.001）。利用ROC曲线，确定α-L-岩藻糖苷酶活性诊断价值的理想临界值为5.6 U/ml。α-L-岩藻糖苷酶活性对结直肠癌的诊断效率高于癌胚抗原（临界值5.0 ng/ml），尤其是对早期肿瘤。