来自转座子Tn10不同tet启动子的纤维单胞菌纤维素酶基因cex和cenA的表达
Expression of the Cellulomonas fimi cellulase genes cex and cenA from the divergent tet promoters of transposon Tn10.
作者信息
Din N, Beck C F, Miller R C, Kilburn D G, Warren R A
机构信息
Department of Microbiology, University of British Columbia, Vancouver, Canada.
出版信息
Arch Microbiol. 1990;153(2):129-33. doi: 10.1007/BF00247809.
PMID:2154164
Abstract
A cartridge was constructed which contained the divergent tet promoters of transposon Tn10 between an exoglucanase gene (cex) and an endoglucanase gene (cenA) of Cellulomonas fimi. When carried in a broad-host-range vector, the cartridge gave expression of cex and cenA in Escherichia coli, Rhodobacter capsulatus and Klebsiella pneumoniae.
摘要
构建了一种盒式结构，其在纤维单胞菌（Cellulomonas fimi）的外切葡聚糖酶基因（cex）和内切葡聚糖酶基因（cenA）之间包含转座子Tn10的发散型tet启动子。当携带在广宿主范围载体中时，该盒式结构在大肠杆菌、荚膜红细菌（Rhodobacter capsulatus）和肺炎克雷伯菌中使cex和cenA得以表达。
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