蛙皮素刺激的瑞士3T3小鼠成纤维细胞中肌醇1,4,5-三磷酸和sn-1,2-二酰基甘油的大规模测量
Mass measurement of inositol 1,4,5-trisphosphate and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 mouse fibroblasts.
作者信息
Cook S J, Palmer S, Plevin R, Wakelam M J
机构信息
Department of Biochemistry, University of Glasgow, Scotland, U.K.
出版信息
Biochem J. 1990 Jan 15;265(2):617-20. doi: 10.1042/bj2650617.
Abstract
Two specific and selective assays were used to measure changes in the mass of Ins(1,4,5)P3 and sn-1,2-diacylglycerol in bombesin-stimulated Swiss 3T3 cells. The results demonstrate that the increase in Ins(1,4,5)P3 was extremely rapid, but transient, returning to basal levels by 30 s. In contrast, the increase in sn-1,2-diacylglycerol was biphasic: the first phase mirrored the transient Ins(1,4,5)P3 response, whereas the second phase was sustained and occurred in the absence of elevated Ins(1,4,5)P3. The possible source of the second phase of diacylglycerol is discussed.
摘要
使用两种特异性和选择性测定法来测量蛙皮素刺激的瑞士3T3细胞中肌醇-1,4,5-三磷酸（Ins(1,4,5)P3）和sn-1,2-二酰甘油的质量变化。结果表明，Ins(1,4,5)P3的增加极其迅速，但具有短暂性，在30秒时恢复到基础水平。相比之下，sn-1,2-二酰甘油的增加是双相的：第一阶段反映了Ins(1,4,5)P3的短暂反应，而第二阶段是持续的，并且在Ins(1,4,5)P3未升高的情况下发生。文中讨论了二酰甘油第二阶段的可能来源。
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