猪睾丸中sn-1-硬脂酰-2-花生四烯酰甘油激酶的纯化与鉴定
Purification and characterization of sn-1-stearoyl-2-arachidonoylglycerol kinase from pig testes.
作者信息
Hodgkin M N, Gardner S D, Rose S, Paterson A, Martin A, Wakelam M J
机构信息
Institute for Cancer Studies, The Medical School, University of Birmingham, Edgbaston, U.K.
出版信息
Biochem J. 1997 Mar 1;322 ( Pt 2)(Pt 2):529-34. doi: 10.1042/bj3220529.
Abstract
1-Stearoyl-2-arachidonoylglycerol (SAG) kinase was identified in the particulate fraction of pig testes. This activity was enriched by hydroxyapatite and blue dye chromatography. The enzyme was selective for polyunsaturated diradylglycerol species and activity was not modulated by other diradylglycerol species or sphingomyelin metabolites. Further purification resulted in the isolation of 55 and 50 kDa proteins that corresponded with SAG kinase activity. These results support the view that the phosphorylation of polyunsaturated diradylglycerol is regulated by structural determinants in the molecule.
摘要
1-硬脂酰-2-花生四烯酰甘油（SAG）激酶是在猪睾丸的微粒体部分中鉴定出来的。这种活性通过羟基磷灰石和蓝色染料层析得到了富集。该酶对多不饱和二酰基甘油种类具有选择性，并且其活性不受其他二酰基甘油种类或鞘磷脂代谢物的调节。进一步纯化导致分离出了与SAG激酶活性相对应的55 kDa和50 kDa蛋白质。这些结果支持了这样一种观点，即多不饱和二酰基甘油的磷酸化受分子中结构决定因素的调节。
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