Rozengurt E, Sinnett-Smith J
Proc Natl Acad Sci U S A. 1983 May;80(10):2936-40. doi: 10.1073/pnas.80.10.2936.
Bombesin is shown to be a potent mitogen for Swiss 3T3 cells. At nanomolar concentrations the peptide markedly enhances the ability of fresh serum to stimulate DNA synthesis in confluent and quiescent cultures of these cells. In the presence of a low concentration (3.5%) of serum, bombesin stimulates 3T3 cell proliferation. In serum-free medium, bombesin induces DNA synthesis in the absence of any other added growth factor; half-maximal effect is obtained at 1 nM. The mitogenic effect of bombesin is dependent on dose and time, is mimicked by litorin, and is markedly potentiated by insulin, colchicine, platelet-derived growth factor, and fibroblast-derived growth factor. These mitogens increase the maximal response elicited by bombesin and decrease the bombesin concentration required to produce half-maximal effect (from 1 nM to 0.3 nM). In contrast, vasopressin, phorbol esters, or cAMP increasing agents fail to enhance the maximal level of DNA synthesis induced by bombesin. Bombesin and litorin may provide useful model peptides for studies on the mechanism(s) by which extracellular ligands control cell proliferation.
蛙皮素被证明是瑞士3T3细胞的一种强效促有丝分裂原。在纳摩尔浓度下，该肽显著增强新鲜血清刺激这些细胞汇合且静止培养物中DNA合成的能力。在低浓度（3.5%）血清存在的情况下，蛙皮素刺激3T3细胞增殖。在无血清培养基中，蛙皮素在没有添加任何其他生长因子的情况下诱导DNA合成；在1 nM时可获得半数最大效应。蛙皮素的促有丝分裂作用取决于剂量和时间，可被利托林模拟，并且明显被胰岛素、秋水仙碱、血小板衍生生长因子和成纤维细胞衍生生长因子增强。这些促有丝分裂原增加了蛙皮素引发的最大反应，并降低了产生半数最大效应所需的蛙皮素浓度（从1 nM降至0.3 nM）。相比之下，血管加压素、佛波酯或cAMP增加剂不能增强蛙皮素诱导的DNA合成的最大水平。蛙皮素和利托林可能为研究细胞外配体控制细胞增殖的机制提供有用的模型肽。
