[羟基红花黄色素A对脂多糖诱导的中性粒细胞活化的抑制作用]

[Inhibitory effect of hydroxysafflor yellow A against PMN activation induced by LPS].

作者信息

Wu Wei, Jin Ming, Tong Jing, Wang Xiao-fei, Zang Bao-xia

机构信息

Beijing Anzhen Hospital, Capital Medical University-Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing 100029, China.

出版信息

Yao Xue Xue Bao. 2011 Feb;46(2):153-7.

PMID:21542285

Abstract

Carthamus tinctorius L. is a traditional Chinese medicine with the effect of promoting blood circulation and removing blood stasis. HSYA (hydroxysafflor yellow A) is the main effective component of Carthamus tinctorius L. In order to study the inhibitory effects of HSYA against PMN (polymorphonuclear) activation induced by LPS (lipopolysaccharide), rabbit PMN adhesion potency which was activated by LPS through colorimetry method was observed. Cellular free calcium concentration was determined by fluorescence spectrophotometry. RT-PCR was applied to study the effect of HSYA on PMN TNF-alpha and IL-6 mRNA expression; The inhibition of HSYA on NF-kappaB activation was monitored with immunofluorescence. The results showed that after treated with HSYA, the increase of adhesion potency (HSYA dose 1.01 x 10(-4) mol x L(-1)), free calcium concentration (HSYA dose 3.1 x 10(-5) mol x L(-1)), TNF-alpha and IL-6 mRNA expression elevation (HSYA dose 5.2 x 10(-1) mol x L(-1)) induced by LPS were inhibited. HSYA can inhibit NF-kappaB p65 subgroup nuclear translocation (HSYA dose 5.2 x 10(-5) mol x L(-1)). It is suggested that HSYA is effective in PMN activation induced by LPS.

摘要

红花是一种具有活血化瘀功效的传统中药。羟基红花黄色素A（HSYA）是红花的主要有效成分。为研究HSYA对脂多糖（LPS）诱导的中性粒细胞（PMN）活化的抑制作用，采用比色法观察LPS激活的兔PMN黏附能力。用荧光分光光度法测定细胞游离钙浓度。应用逆转录聚合酶链反应（RT-PCR）研究HSYA对PMN肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）mRNA表达的影响；用免疫荧光法监测HSYA对核因子-κB（NF-κB）活化的抑制作用。结果显示，HSYA处理后，LPS诱导的黏附能力增加（HSYA剂量为1.01×10⁻⁴mol·L⁻¹）、游离钙浓度升高（HSYA剂量为3.1×10⁻⁵mol·L⁻¹）、TNF-α和IL-6 mRNA表达上调（HSYA剂量为5.2×10⁻¹mol·L⁻¹）均受到抑制。HSYA可抑制NF-κB p65亚组核转位（HSYA剂量为5.2×10⁻⁵mol·L⁻¹）。提示HSYA对LPS诱导的PMN活化具有抑制作用。